Rabbit anti-Human, Mouse DIS3L2 Polyclonal Antibody | anti-DIS3L2 antibody

DIS3L2 Antibody

Cat. Num. AAA9629868

• Gene Names: DIS3L2; FAM6A; PRLMNS; hDIS3L2
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DIS3L2; Polyclonal Antibody; DIS3L2 Antibody; DI3L2_HUMAN; DIS3 L2; DIS3 mitotic control homolog (S. cerevisiae) like 2; DIS3 mitotic control homolog like 2; DIS3-like exonuclease 2; Dis3l2; FLJ36974; MGC42174; anti-DIS3L2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: DIS3L2 Antibody detects endogenous levels of DIS3L2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-DIS3L2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human DIS3L2.
• Conjugation: Unconjugated
• Similarity: Specifically recognizes and binds polyuridylated RNAs via 3 RNA-binding regions (named U-zone 1, U-zone 2 and U-zone 3) that form an open funnel on one face of the catalytic domain, allowing RNA to navigate a path to the active site. Belongs to the RNR ribonuclease family. DIS3L2 subfamily.
• Subunit Structure: Interacts with XRN1.
• Function: 3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-DIS3L2 antibody

Cytoplasm; P-body; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 134288890
• NCBI GeneID: 129563
• NCBI Accession #: NP_689596
• NCBI GenBank Nucleotide #: NM_152383.4
• UniProt Accession #: Q8IYB7; Q53S79; Q580W6; Q5XKH0; Q69YG5; Q6AW99; Q7Z4T6; Q8N9K9
• Molecular Weight: Predicted: 97 kDa; Observed: 94 kDa
• NCBI Official Full Name: DIS3-like exonuclease 2 isoform 1
• NCBI Official Synonym Full Names: DIS3 like 3'-5' exoribonuclease 2
• NCBI Official Symbol: DIS3L2
• NCBI Official Synonym Symbols: FAM6A; PRLMNS; hDIS3L2
• NCBI Protein Information: DIS3-like exonuclease 2
• UniProt Protein Name: DIS3-like exonuclease 2
• Protein Family: DIS3-like exonuclease
• UniProt Gene Name: DIS3L2
• UniProt Synonym Gene Names: hDIS3L2
• UniProt Entry Name: DI3L2_HUMAN

NCBI Description

The protein encoded by this gene is similar in sequence to 3'/5' exonucleolytic subunits of the RNA exosome. The exosome is a large multimeric ribonucleotide complex responsible for degrading various RNA substrates. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Mar 2012]

Uniprot Description

DIS3L2: Ribonuclease that plays a critical role in RNA metabolism. It is essential for correct mitosis, and negatively regulates cell proliferation. Defects in DIS3L2 are the cause of Perlman syndrome (PRLMNS). An autosomal recessive congenital overgrowth syndrome. Affected children are large at birth, are hypotonic, and show organomegaly, characteristic facial dysmorphisms (inverted V-shaped upper lip, prominent forehead, deep-set eyes, broad and flat nasal bridge, and low-set ears), renal anomalies (nephromegaly and hydronephrosis), frequent neurodevelopmental delay, and high neonatal mortality. Perlman syndrome is associated with a high risk of Wilms tumor. Histologic examination of the kidneys in affected children shows frequent nephroblastomatosis, which is a precursor lesion for Wilms tumor. Belongs to the RNR ribonuclease family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.13.-; Ribonuclease; Tumor suppressor

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cytoplasm; nuclear exosome (RNase complex); polysome

Molecular Function: 3'-5'-exoribonuclease activity; magnesium ion binding; poly(U) binding; protein binding; ribonuclease activity

Biological Process: cell division; mitosis; mitotic sister chromatid separation; mRNA catabolic process, exonucleolytic; negative regulation of cell proliferation; rRNA catabolic process; rRNA processing; stem cell maintenance

Disease: Perlman Syndrome