Rabbit anti-Human DIRAS1 Polyclonal Antibody | anti-DIRAS1 antibody

DIRAS1, ID (DIRAS1, GBTS1, RIG, GTP-binding protein Di-Ras1, Distinct subgroup of the Ras family member 1, Ras-related inhibitor of cell growth, Small GTP-binding tumor suppressor 1) (FITC)

Cat. Num. AAA6292193

• Gene Names: DIRAS1; RIG; GBTS1; Di-Ras1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DIRAS1; Polyclonal Antibody; ID (DIRAS1; GBTS1; RIG; GTP-binding protein Di-Ras1; Distinct subgroup of the Ras family member 1; Ras-related inhibitor of cell growth; Small GTP-binding tumor suppressor 1) (FITC); Small GTP-binding tumor suppressor 1; anti-DIRAS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DIRAS1 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DIRAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115~145 amino acids from the Central region of human DIRAS1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DIRAS1 Antibody (Center) in CEM cell line lysates (35ug/lane). DIRAS1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with DIRAS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DIRAS1 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DIRAS1 antibody

DIRAS1 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.

Product Categories/Family for anti-DIRAS1 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Kimura, K., et al. Genome Res. 16(1):55-65(2006). Kontani, K., et al. J. Biol. Chem. 277(43):41070-41078(2002). Ellis, C.A., et al. Proc. Natl. Acad. Sci. U.S.A. 99(15):9876-9881(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 21553323
• NCBI GeneID: 148252
• NCBI Accession #: NP_660156.1
• NCBI GenBank Nucleotide #: NM_145173.3
• UniProt Accession #: O95057
• Molecular Weight: 22,329 Da
• NCBI Official Full Name: GTP-binding protein Di-Ras1
• NCBI Official Synonym Full Names: DIRAS family, GTP-binding RAS-like 1
• NCBI Official Symbol: DIRAS1
• NCBI Official Synonym Symbols: RIG; GBTS1; Di-Ras1
• NCBI Protein Information: GTP-binding protein Di-Ras1; ras-related inhibitor of cell growth; small GTP-binding tumor suppressor 1; distinct subgroup of the Ras family member 1
• UniProt Protein Name: GTP-binding protein Di-Ras1
• Protein Family: GTP-binding protein
• UniProt Gene Name: DIRAS1
• UniProt Synonym Gene Names: GBTS1; RIG; Rig
• UniProt Entry Name: DIRA1_HUMAN

NCBI Description

DIRAS1 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.[supplied by OMIM, Apr 2004]

Uniprot Description

DIRAS1: Displays low GTPase activity and exist predominantly in the GTP-bound form. Belongs to the small GTPase superfamily. Di-Ras family.

Protein type: G protein, monomeric, di-Ras; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: plasma membrane

Molecular Function: GTPase activity; GTP binding; mitogen-activated protein kinase binding

Biological Process: positive regulation of MAP kinase activity; metabolic process; small GTPase mediated signal transduction