Western Blot (WB)
(Western blot analysis of DIPA Antibody (Center) in HepG2 cell line lysates (35ug/lane). DIPA (arrow) was detected using the purified Pab.)
Rabbit anti-Human DIPA Polyclonal Antibody | anti-DIPA antibody
DIPA, ID (CCDC85B, DIPA, Coiled-coil domain-containing protein 85B, Hepatitis delta antigen-interacting protein A) (APC)
Gene Names
CCDC85B; DIPA
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Synonyms
DIPA; Polyclonal Antibody; ID (CCDC85B; Coiled-coil domain-containing protein 85B; Hepatitis delta antigen-interacting protein A) (APC); CCDC85B; Hepatitis delta antigen-interacting protein A; anti-DIPA antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human DIPA
Purity/Purification
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-DIPA antibody
Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
ELISA: 1:1,000
IHC: 1:50-100
WB: 1:50-100
Applications are based on unconjugated antibody.
Immunogen
DIPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75~105 amino acids from the Center region of human DIPA.
Conjugate
APC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of DIPA Antibody (Center) in HepG2 cell line lysates (35ug/lane). DIPA (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DIPA Antibody (Center) in HepG2 cell line lysates (35ug/lane). DIPA (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with DIPA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human skeletal muscle reacted with DIPA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(DIPA Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DIPA Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-DIPA antibody
DIPA functions as a transcriptional repressor. It may inhibit the activity of CTNNB1 in a TP53-dependent manner and thus regulate cell growth. It may function in adipocyte differentiation, negatively regulating mitotic clonal expansion.
Product Categories/Family for anti-DIPA antibody
References
Bezy, O., et.al., J. Biol. Chem. 280 (12), 11432-11438 (2005). Du, X., et.al., Exp. Mol. Pathol. 81 (3), 184-190 (2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,091 Da
NCBI Official Full Name
coiled-coil domain-containing protein 85B
NCBI Official Synonym Full Names
coiled-coil domain containing 85B
NCBI Official Symbol
CCDC85B
NCBI Official Synonym Symbols
DIPA
NCBI Protein Information
coiled-coil domain-containing protein 85B; delta-interacting protein A; hepatitis delta antigen interacting protein A; hepatitis delta antigen-interacting protein A
UniProt Protein Name
Coiled-coil domain-containing protein 85B
UniProt Gene Name
CCDC85B
UniProt Synonym Gene Names
DIPA; Delta-interacting protein A
UniProt Entry Name
CC85B_HUMAN
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Product Notes
The DIPA ccdc85b (Catalog #AAA6292180) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DIPA, ID (CCDC85B, DIPA, Coiled-coil domain-containing protein 85B, Hepatitis delta antigen-interacting protein A) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DIPA can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 ELISA: 1:1,000 IHC: 1:50-100 WB: 1:50-100 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DIPA ccdc85b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DIPA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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