Rabbit anti-Human Dickkopf Related Protein 1 (DKK1) Polyclonal Antibody | anti-DKK1 antibody

Polyclonal Antibody to Dickkopf Related Protein 1 (DKK1)

Cat. Num. AAA2006990

• Gene Names: DKK1; SK; DKK-1
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Dickkopf Related Protein 1 (DKK1); Polyclonal Antibody; Polyclonal Antibody to Dickkopf Related Protein 1 (DKK1); anti-DKK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against DKK1. It has been selected for its ability to recognize DKK1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.; MGHHHHHHSGSEF-LNSVLNSN AIKNLPPPLG GAAGHPGSAV SAAPGILYPG GNKYQTIDNY QPYPCAEDEE CGTDEYCASP TRGGDAGVQI CLACRKRRKR CMRHAMCCPG NYCKNGICVS SDQNHFRGEI EETITESFGN DHSTLDGYSR RTTLSSKMYH TKGQEGSVCL RSSDCASGLC CARHFWSKIC KPVLKEGQVC TKHRRKGSHG LEIFQRCYCG EGLSCRIQKD HHQASNSSRL HTCQRH
• Sequence Length: 266

• Applicable Applications for anti-DKK1 antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant DKK1 (Leu33~His266) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2046777
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Porcine Brain Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant DKK1, Human.)

NCBI and Uniprot Product Information

• NCBI GI #: 7110719
• NCBI GeneID: 22943
• NCBI Accession #: NP_036374.1
• NCBI GenBank Nucleotide #: NM_012242.3
• UniProt Accession #: O94907; B2RC19
• Molecular Weight: 28,672 Da
• NCBI Official Full Name: dickkopf-related protein 1
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 1
• NCBI Official Symbol: DKK1
• NCBI Official Synonym Symbols: SK; DKK-1
• NCBI Protein Information: dickkopf-related protein 1
• UniProt Protein Name: Dickkopf-related protein 1
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK1
• UniProt Synonym Gene Names: Dickkopf-1; Dkk-1; hDkk-1

NCBI Description

This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. [provided by RefSeq, Sep 2017]

Uniprot Description

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: early endosome membrane; extracellular region; extracellular space; plasma membrane

Molecular Function: growth factor activity; low-density lipoprotein receptor binding; protein binding; receptor antagonist activity; signal transducer activity

Biological Process: cellular morphogenesis during differentiation; embryonic limb morphogenesis; endoderm formation; forebrain development; hair follicle development; learning and/or memory; limb development; mesoderm formation; negative regulation of apoptosis; negative regulation of BMP signaling pathway; negative regulation of mesodermal cell fate specification; negative regulation of ossification; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein binding; negative regulation of skeletal muscle development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt signaling pathway; positive regulation of JUN kinase activity; positive regulation of Wnt signaling pathway, calcium modulating pathway; regulation of endodermal cell fate specification; regulation of receptor internalization; regulation of synapse organization; regulation of synaptic transmission, glutamatergic; response to retinoic acid