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Western Blot (WB) (Western blot analysis of extracts from HeLa cells, Jurkat cells and HepG2 cells, using DHX8 antibody.)

Rabbit anti-Human DHX8 Polyclonal Antibody | anti-DHX8 antibody

DHX8 Antibody

Gene Names
DHX8; DDX8; HRH1; PRP22; PRPF22
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DHX8; Polyclonal Antibody; DHX8 Antibody; DDX8; HRH1; PRP22; PRPF22; anti-DHX8 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total DHX8 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
1181
Applicable Applications for anti-DHX8 antibody
Western Blot (WB)
Application Notes
Western blotting: 1:500~1:3000
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from internal of human DHX8.
Target Name
DHX8
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, Jurkat cells and HepG2 cells, using DHX8 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HeLa cells, Jurkat cells and HepG2 cells, using DHX8 antibody.)
Related Product Information for anti-DHX8 antibody
Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome.Ono Y., Mol. Cell. Biol. 14:7611-7620(1994).Ohno M., Genes Dev. 10:997-1007(1996).Jurica M.S., RNA 8:426-439(2002).
Product Categories/Family for anti-DHX8 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
139,315 Da
NCBI Official Full Name
ATP-dependent RNA helicase DHX8 isoform 2
NCBI Official Synonym Full Names
DEAH (Asp-Glu-Ala-His) box polypeptide 8
NCBI Official Symbol
DHX8
NCBI Official Synonym Symbols
DDX8; HRH1; PRP22; PRPF22
NCBI Protein Information
ATP-dependent RNA helicase DHX8
UniProt Protein Name
ATP-dependent RNA helicase DHX8
UniProt Gene Name
DHX8
UniProt Synonym Gene Names
DDX8
UniProt Entry Name
DHX8_HUMAN

NCBI Description

This gene is a member of the DEAH box polypeptide family. The encoded protein contains the DEAH (Asp-Glu-Ala-His) motif which is characteristic of all DEAH box proteins, and is thought to function as an ATP-dependent RNA helicase that regulates the release of spliced mRNAs from spliceosomes prior to their export from the nucleus. This protein may be required for the replication of human immunodeficiency virus type 1 (HIV-1). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Uniprot Description

DDX8: Facilitates nuclear export of spliced mRNA by releasing the RNA from the spliceosome. Belongs to the DEAD box helicase family. DEAH subfamily. DDX8/PRP22 sub-subfamily.

Protein type: RNA splicing; RNA-binding; Spliceosome; Helicase; EC 3.6.4.13; RNA processing

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; spliceosome; cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; ATP binding; ATP-dependent RNA helicase activity

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing

Research Articles on DHX8

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Product Notes

The DHX8 dhx8 (Catalog #AAA9405573) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHX8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DHX8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blotting: 1:500~1:3000. Researchers should empirically determine the suitability of the DHX8 dhx8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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