Rabbit anti-Mouse DHX32 Polyclonal Antibody | anti-DHX32 antibody

DHX32, ID (DHX32, DDX32, Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32, DEAD/H box 32, DEAD/H helicase-like protein 1, DEAH box protein 32, HuDDX32) (Azide free) (HRP)

Cat. Num. AAA6292106

• Gene Names: DHX32; DDX32; DHLP1
• Reactivity: Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DHX32; Polyclonal Antibody; ID (DHX32; DDX32; Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32; DEAD/H box 32; DEAD/H helicase-like protein 1; DEAH box protein 32; HuDDX32) (Azide free) (HRP); HuDDX32; anti-DHX32 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DHX32 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DHX32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-371 amino acids from the Central region of human DHX32.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DHX32 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the DHX32 antibody detected the DHX32 protein (arrow).)

Immunohistochemistry (IHC)

(DHX32 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DHX32 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-DHX32 antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined.

Product Categories/Family for anti-DHX32 antibody

Antibodies; Splicing Factors

References

Huang, C., et al. J. Exp. Clin. Cancer Res. 28, 11 (2009): Chen, Y., et al. Exp. Mol. Pathol. 82(3):256-261(2007). Alli, Z., et al. Anticancer Res. 27 (1A), 373-377 (2007): Alli, Z., et al. Exp. Mol. Pathol. 81(3):245-248(2006). Alli, Z., et al. Cell. Immunol. 237(2):141-146(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 20336300
• NCBI GeneID: 55760
• NCBI Accession #: NP_060650.2
• NCBI GenBank Nucleotide #: NM_018180.2
• UniProt Accession #: Q7L7V1; Q49AG5; Q5T3L0; Q5T3L5; Q96NY1; Q9BUN0; Q9H769; Q9NSL5; Q9NV74; Q9NVJ7; A8MSV2; D3DRF9
• Molecular Weight: 75,088 Da
• NCBI Official Full Name: putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
• NCBI Official Synonym Full Names: DEAH (Asp-Glu-Ala-His) box polypeptide 32
• NCBI Official Symbol: DHX32
• NCBI Official Synonym Symbols: DDX32; DHLP1
• NCBI Protein Information: putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32; huDDX32; DEAD/H box 32; DEAH box protein 32; DEAD/H helicase-like protein 1; DEAD/H helicase-like protein-1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 32
• UniProt Protein Name: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32
• Protein Family: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase
• UniProt Gene Name: DHX32
• UniProt Synonym Gene Names: DDX32; DHLP1

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

DHX32: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined. [provided by RefSeq, Jul 2008]

Protein type: EC 3.6.4.13; Helicase

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: cytoplasm; mitochondrion; spliceosomal complex

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; protein binding; RNA binding

Biological Process: mRNA splicing, via spliceosome