Rabbit DHX29 Polyclonal Antibody | anti-DHX29 antibody

DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29, DDX29) (FITC)

Cat. Num. AAA6414047

• Gene Names: DHX29; DDX29
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: DHX29; Polyclonal Antibody; DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29; DDX29) (FITC); anti-DHX29 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes DHX29. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)
• Sequence Length: 1369

• Applicable Applications for anti-DHX29 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human DHX29.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for anti-DHX29 antibody

Antibodies; DEAH Box Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 110278938
• NCBI GeneID: 54505
• UniProt Accession #: Q7Z478
• Molecular Weight: 155
• NCBI Official Full Name: ATP-dependent RNA helicase DHX29
• NCBI Official Synonym Full Names: DExH-box helicase 29
• NCBI Official Symbol: DHX29
• NCBI Official Synonym Symbols: DDX29
• NCBI Protein Information: ATP-dependent RNA helicase DHX29
• UniProt Protein Name: ATP-dependent RNA helicase DHX29
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DHX29
• UniProt Synonym Gene Names: DDX29
• UniProt Entry Name: DHX29_HUMAN

NCBI Description

This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection. This protein may also play a role in sensing virally derived cytosolic nucleic acids. Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells. [provided by RefSeq, Sep 2016]

Uniprot Description

DDX29: ATP-binding RNA helicase involved in translation initiation. Required for efficient initiation on mammalian mRNAs with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity. Belongs to the DEAD box helicase family. DEAH subfamily.

Protein type: Translation; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: mitochondrion; nucleus

Molecular Function: translation initiation factor activity; ATP-dependent RNA helicase activity; ribosomal small subunit binding; ATP binding

Biological Process: RNA processing; translational initiation