Immunohistochemistry (IHC)
(MBS9603298 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit DHRS3 Polyclonal Antibody | anti-DHRS3 antibody
DHRS3 Antibody
Gene Names
DHRS3; SDR1; RDH17; Rsdr1; DD83.1; SDR16C1; retSDR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DHRS3; Polyclonal Antibody; DHRS3 Antibody; DD83.1; Dehydrogenase/reductase (SDR family) member 3; DHRS3_HUMAN; EC 1.1.1.300; MGC125166; RDH17; Retinal short chain dehydrogenase/reductase 1; Retinal short-chain dehydrogenase/reductase 1; retSDR1; Rsdr1; SDR family; member 3; SDR1; SDR16C1; Short-chain dehydrogenase/reductase 3; Short-chain dehydrogenase/reductase family; anti-DHRS3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DHRS3 antibody detects endogenous levels of DHRS3.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
302
Applicable Applications for anti-DHRS3 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human DHRS3.
Subcellular Location
Membrane.
Tissue Specificity
Widely expressed with highest levels found in heart, placenta, lung, liver, kidney, pancreas, thyroid, testis, stomach, trachea and spinal cord. Lower levels found in skeletal muscle, intestine and lymph node. No expression detected in brain. In the retina, expressed in cone but not rod outer segments.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (89%), Xenopus (88%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603298 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603298 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of A375 cells, using DHRS3 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of A375 cells, using DHRS3 antibody.)
Related Product Information for anti-DHRS3 antibody
Function: Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 34 kDa
Predicted: 34 kDa
Predicted: 34 kDa
NCBI Official Full Name
short-chain dehydrogenase/reductase 3 isoform 1
NCBI Official Synonym Full Names
dehydrogenase/reductase 3
NCBI Official Symbol
DHRS3
NCBI Official Synonym Symbols
SDR1; RDH17; Rsdr1; DD83.1; SDR16C1; retSDR1
NCBI Protein Information
short-chain dehydrogenase/reductase 3
UniProt Protein Name
Short-chain dehydrogenase/reductase 3
Protein Family
UniProt Gene Name
DHRS3
UniProt Synonym Gene Names
RDH17; SDR16C1; retSDR1
NCBI Description
Short-chain dehydrogenases/reductases (SDRs), such as DHRS3, catalyze the oxidation/reduction of a wide range of substrates, including retinoids and steroids (Haeseleer and Palczewski, 2000 [PubMed 10800688]).[supplied by OMIM, Jun 2009]
Uniprot Description
Catalyzes the reduction of all-trans-retinal to all-trans-retinol in the presence of NADPH.
Research Articles on DHRS3
Similar Products
Product Notes
The DHRS3 dhrs3 (Catalog #AAA9603298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DHRS3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DHRS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the DHRS3 dhrs3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHRS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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