Rabbit DHPS/DHS Polyclonal Antibody | anti-DHPS antibody

Anti-DHPS/DHS Antibody

Cat. Num. AAA1753490

• Gene Names: DHPS; DS; DHS; MIG13
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: DHPS/DHS; Polyclonal Antibody; Anti-DHPS/DHS Antibody; DHPS; DS; Deoxyhypusine synthase; DHS; EC 2. 5. 1. 46; deoxyhypusine synthase; anti-DHPS antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rabbit IgG polyclonal antibody for DHPS/DHS detection.
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized. Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

• Applicable Applications for anti-DHPS antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA)
• Application Notes: WB: 0.25-0.5ug/ml|Human, Mouse, Rat|; IHC-P: 2-5ug/ml|Human|; ICC/IF: 5ug/ml|Human|; FC/FACS/FCM: 1-3ug/1x106 cells|Human|; Direct ELISA: 0.1-0.5ug/ml|Human|
• Immunogen: E Coli-derived human DHPS recombinant protein (Position: F54-K358).
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Recommended Detection Systems
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Related Product Information for anti-DHPS antibody

Deoxyhypusine synthase is an enzyme that in humans is encoded by the DHPS gene. This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

References

1. Bevec, D., Kappel, B., Jaksche, H., Csonga, R., Hauber, J., Klier, H., Steinkasserer, A. Molecular characterization of a cDNA encoding functional human deoxyhypusine synthase and chromosomal mapping of the corresponding gene locus. FEBS Lett. 378: 195-198, 1996.
2. Ganapathi, M., Padgett, L. R., Yamada, K., Devinsky, O., Willaert, R., Person, R., Au, P. -Y. B., Tagoe, J., McDonald, M., Karlowicz, D., Wolf, B., Lee, J., and 10 others. Recessive rare variants in deoxyhypusine synthase, an enzyme involved in the synthesis of hypusine, are associated with a neurodevelopmental disorder. Am. J. Hum. Genet. 104: 287-298, 2019.
3. Hauber, I., Bevec, D., Heukeshoven, J., Kratzer, F., Horn, F., Choidas, A., Harrer, T., Hauber, J. Identification of cellular deoxyhypusine synthase as a novel target for antiretroviral therapy. J. Clin. Invest. 115: 76-85, 2005.

NCBI and Uniprot Product Information

• NCBI GI #: 4503325
• NCBI GeneID: 1725
• NCBI Accession #: NP_001921.1
• NCBI GenBank Nucleotide #: NM_001930.3
• UniProt Accession #: P49366; Q13184; Q13276; Q9UDG0; A8K688
• Molecular Weight: 40,971 Da
• NCBI Official Full Name: deoxyhypusine synthase isoform a
• NCBI Official Synonym Full Names: deoxyhypusine synthase
• NCBI Official Symbol: DHPS
• NCBI Official Synonym Symbols: DS; DHS; MIG13
• NCBI Protein Information: deoxyhypusine synthase; migration-inducing gene 13
• UniProt Protein Name: Deoxyhypusine synthase
• UniProt Gene Name: DHPS
• UniProt Synonym Gene Names: DS; DHS
• UniProt Entry Name: DHYS_HUMAN

NCBI Description

This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011]

Uniprot Description

DHPS: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. Belongs to the deoxyhypusine synthase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 2.5.1.46; Transferase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cytosol

Molecular Function: protein binding; deoxyhypusine synthase activity

Biological Process: cellular protein metabolic process; translation; positive regulation of cell proliferation; positive regulation of T cell proliferation; hypusine biosynthetic process from peptidyl-lysine; post-translational protein modification; glucose homeostasis; spermidine catabolic process to deoxyhypusine, using deoxyhypusine synthase; protein homotetramerization