Rabbit anti-Human, Mouse DHCR24 Polyclonal Antibody | anti-DHCR24 antibody

DHCR24, NT (24-dehydrocholesterol Reductase, 3-beta-hydroxysterol delta-24-reductase, Seladin-1, Diminuto/Dwarf1 Homolog, DHCR24, KIAA0018) (APC)

Cat. Num. AAA6472372

• Gene Names: DHCR24; DCE; SELADIN1; Nbla03646; seladin-1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: DHCR24; Polyclonal Antibody; NT (24-dehydrocholesterol Reductase; 3-beta-hydroxysterol delta-24-reductase; Seladin-1; Diminuto/Dwarf1 Homolog; KIAA0018) (APC); anti-DHCR24 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DHCR24. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DHCR24 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human DHCR24 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-DHCR24 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). DHCR24 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of anti-DHCR24 Antibody (N-term) in mouse liver tissue lysates (35ug/lane). DHCR24 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-DHCR24 antibody

Antibodies; Proteins

References

1. Wang, Y., Mol. Pharmacol. 74(6), 1716-1721 (2008). 2. Bonaccorsi, L., Lab. Invest. 88(10), 1049-1056 (2008). 3. Cecchi, C., J. Cell. Mol. Med. 12(5B), 1990-2002 (2008).

NCBI and Uniprot Product Information

• NCBI GI #: 13375618
• NCBI GeneID: 1718
• NCBI Accession #: NP_055577
• NCBI GenBank Nucleotide #: NM_014762
• UniProt Accession #: Q15392; Q9HBA8; B7Z817; D3DQ51
• Molecular Weight: 54,750 Da
• NCBI Official Full Name: delta(24)-sterol reductase
• NCBI Official Synonym Full Names: 24-dehydrocholesterol reductase
• NCBI Official Symbol: DHCR24
• NCBI Official Synonym Symbols: DCE; SELADIN1; Nbla03646; seladin-1
• NCBI Protein Information: delta(24)-sterol reductase; delta(24)-sterol reductase; 3 beta-hydroxysterol delta 24-reductase; 3-beta-hydroxysterol delta-24-reductase; desmosterol-to-cholesterol enzyme; diminuto/dwarf1 homolog; seladin 1; selective AD indicator 1
• UniProt Protein Name: Delta(24)-sterol reductase
• Protein Family: Delta(24)-sterol reductase
• UniProt Gene Name: DHCR24
• UniProt Synonym Gene Names: KIAA0018
• UniProt Entry Name: DHC24_HUMAN

Uniprot Description

Diminuto: the enzyme 3-beta-hydroxysterol delta-24-reductase (DHCR24), a member of the flavin adenine dinucleotide (FAD)-dependent oxidoreductases, catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate. Undetectable in blood cells. Overexpressed in the adenoma tissue of patients with Cushing's syndrome. May be involved in the molecular events of adrenocortical tumorigenesis by facilitating steroid synthesis and cell growth.

Protein type: Oxidoreductase; Lipid Metabolism - steroid biosynthesis; Membrane protein, integral; EC 1.3.1.72

Chromosomal Location of Human Ortholog: 1p32.3

Cellular Component: Golgi membrane; endoplasmic reticulum membrane; cytoskeleton; membrane; endoplasmic reticulum; integral to membrane; cytosol; nucleus

Molecular Function: delta24(24-1) sterol reductase activity; UDP-N-acetylmuramate dehydrogenase activity; enzyme binding; FAD binding; peptide antigen binding; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; delta24-sterol reductase activity

Biological Process: skin development; tissue development; negative regulation of cell proliferation; amyloid precursor protein catabolic process; protein localization; apoptosis; Ras protein signal transduction; male genitalia development; response to hormone stimulus; negative regulation of caspase activity; response to oxidative stress; cell cycle arrest; cholesterol biosynthetic process; plasminogen activation; negative regulation of apoptosis

Disease: Desmosterolosis