Typical Testing Data/Standard Curve (for reference only)
Human B4GALT6/Beta-1,4-Galactosyltransferase 6 ELISA Kit | B4GALT6 elisa kit
Human B4GALT6/Beta-1,4-Galactosyltransferase 6 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene is a lactosylceramide synthase important for glycolipid biosynthesis. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Required for the biosynthesis of glycosphingolipids. Ref.5 Ref.6
Catalytic activity: UDP-galactose + glucosylceramide = UDP + lactosylceramide. Ref.2 Ref.5 Ref.6UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide. Ref.2 Ref.5 Ref.6
Cofactor: Manganese or magnesium. Ref.5
Enzyme regulation: Inhibited by EDTA
By similarity.
Pathway: Protein modification; protein glycosylation.
Subcellular location: Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Trans cisternae of Golgi stack.
Tissue specificity: High expression in brain and adrenal gland, lower in liver, lung, colon and peripheral white blood cells.
Sequence similarities: Belongs to the glycosyltransferase 7 family.
Biophysicochemical propertiesKinetic parameters:KM=3 µM for glucosylceramide Ref.5KM=0.5 µM for UDP-galactoseVmax=0.06 nmol/h/mg enzyme toward glucosylceramideVmax=86 nmol/h/mg enzyme toward UDP-galactose
Research Articles on B4GALT6
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Product Notes
The Human B4GALT6 b4galt6 (Catalog #AAA2890992) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2890992 ELISA Kit recognizes Human B4GALT6. It is sometimes possible for the material contained within the vial of "B4GALT6/Beta-1,4-Galactosyltransferase 6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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