Rabbit anti-Human DGKD Polyclonal Antibody | anti-DGKD antibody

DGKD Antibody (C-term)

Cat. Num. AAA9204920

• Gene Names: DGKD; dgkd-2; DGKdelta
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DGKD; Polyclonal Antibody; DGKD Antibody (C-term); Diacylglycerol kinase delta; DAG kinase delta; 130 kDa diacylglycerol kinase; Diglyceride kinase delta; DGK-delta; KIAA0145; anti-DGKD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DGKD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1061-1091 amino acids from the C-terminal region of human DGKD.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 1061-1091
• Sequence Length: 1,170

• Applicable Applications for anti-DGKD antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-anti-DGK delta Pab in Jurkat cell line lysates (35ug/lane). DGK delta (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-DGKD antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).

Product Categories/Family for anti-DGKD antibody

Signal Transduction

References

Sakane, F., et al., J. Biol. Chem. 271(14):8394-8401 (1996). Nagase, T., et al., DNA Res. 2(4):167-174 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 25777596
• NCBI GeneID: 8527
• NCBI Accession #: NP_003639.2; NP_690618.2
• NCBI GenBank Nucleotide #: NM_003648.2
• UniProt Accession #: Q16760; Q14158; Q6PK55; Q8NG53
• Molecular Weight: 134525
• NCBI Official Full Name: diacylglycerol kinase delta isoform 1
• NCBI Official Synonym Full Names: diacylglycerol kinase, delta 130kDa
• NCBI Official Symbol: DGKD
• NCBI Official Synonym Symbols: dgkd-2; DGKdelta
• NCBI Protein Information: diacylglycerol kinase delta
• UniProt Protein Name: Diacylglycerol kinase delta
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKD
• UniProt Synonym Gene Names: KIAA0145; DAG kinase delta; DGK-delta
• UniProt Entry Name: DGKD_HUMAN

NCBI Description

This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKD: May function as signaling molecule. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, lipid; Motility/polarity/chemotaxis; Lipid Metabolism - glycerophospholipid; EC 2.7.1.107; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane

Molecular Function: diacylglycerol binding; protein binding; protein homodimerization activity; protein heterodimerization activity; metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; second-messenger-mediated signaling; platelet activation; multicellular organismal development; endocytosis; signal transduction; response to organic substance; protein transport; protein kinase C activation; diacylglycerol metabolic process; blood coagulation; cell growth; phosphorylation; protein homooligomerization