Rabbit anti-Human, Mouse DGKD Polyclonal Antibody | anti-DGKD antibody

DGKD, CT (Diacylglycerol Kinase delta, Diglyceride Kinase delta, DGK-delta, DAG Kinase delta, 130kD Diacylglycerol Kinase, KIAA0145) (Biotin)

Cat. Num. AAA6472407

• Gene Names: DGKD; dgkd-2; DGKdelta
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: DGKD; Polyclonal Antibody; CT (Diacylglycerol Kinase delta; Diglyceride Kinase delta; DGK-delta; DAG Kinase delta; 130kD Diacylglycerol Kinase; KIAA0145) (Biotin); anti-DGKD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DGKD. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DGKD antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human DGKD (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-anti-DGK delta Pab in Jurkat cell line lysates (35ug/lane). DGK delta (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-DGKD antibody

Antibodies; Protein Kinases

References

1. Sakane, F., et al., J. Biol. Chem. 271(14):8394-8401 (1996). 2. Nagase, T., et al., DNA Res. 2(4):167-174 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 25777596
• NCBI GeneID: 8527
• NCBI Accession #: NP_003639.2
• NCBI GenBank Nucleotide #: NM_003648.2
• UniProt Accession #: Q16760; Q14158; Q6PK55; Q8NG53
• Molecular Weight: 134,525 Da
• NCBI Official Full Name: diacylglycerol kinase delta isoform 1
• NCBI Official Synonym Full Names: diacylglycerol kinase, delta 130kDa
• NCBI Official Symbol: DGKD
• NCBI Official Synonym Symbols: dgkd-2; DGKdelta
• NCBI Protein Information: diacylglycerol kinase delta; DGK-delta; DAG kinase delta; diglyceride kinase delta
• UniProt Protein Name: Diacylglycerol kinase delta
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKD
• UniProt Synonym Gene Names: KIAA0145; DAG kinase delta; DGK-delta
• UniProt Entry Name: DGKD_HUMAN

NCBI Description

This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKD: May function as signaling molecule. Belongs to the eukaryotic diacylglycerol kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid; Kinase, lipid; EC 2.7.1.107; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cytoplasmic membrane-bound vesicle; cytoplasm; plasma membrane

Molecular Function: diacylglycerol binding; protein binding; protein homodimerization activity; protein heterodimerization activity; metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; second-messenger-mediated signaling; platelet activation; multicellular organismal development; endocytosis; signal transduction; response to organic substance; protein transport; protein kinase C activation; diacylglycerol metabolic process; blood coagulation; cell growth; phosphorylation; protein homooligomerization