Western Blot (WB)
(Western blot analysis of DGK iota expression in HEK293T (A), Hela (B), mouse brain (C) whole cell lysates.)
Rabbit anti-Human, Mouse DGK iota Polyclonal Antibody | anti-DGKI antibody
Anti-DGK iota Antibody
Western Blot (WB)
(Western blot analysis of DGK iota expression in HEK293T (A), Hela (B), mouse brain (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of DGK iota expression in HEK293T (A), Hela (B), mouse brain (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of DGK iota staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of DGK iota staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of DGK iota staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of DGK iota staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008]
Uniprot Description
DGKI: a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008]
Protein type: EC 2.7.1.107; Kinase, lipid; Motility/polarity/chemotaxis; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid
Chromosomal Location of Human Ortholog: 7q32.3-q33
Cellular Component: cell soma; cytoplasm; cytosol; dendritic spine; nerve terminal; nucleoplasm; nucleus; perinuclear region of cytoplasm; plasma membrane; presynaptic active zone; protein complex; synapse; synaptic vesicle
Molecular Function: ATP binding; diacylglycerol kinase activity; GTPase inhibitor activity; metal ion binding; protein binding; Ras GTPase binding
Biological Process: blood coagulation; habituation; neurotransmitter secretion; phosphorylation; platelet activation; positive regulation of Ras protein signal transduction; protein kinase C activation; regulation of excitatory postsynaptic membrane potential; regulation of synaptic transmission, glutamatergic
Research Articles on DGKI
Similar Products
Product Notes
The DGKI dgki (Catalog #AAA8242629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DGK iota Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DGK iota can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). Western Blot: (1/500 - 1/1000); Immunohistochemistry: (1/100 - 1/200); Immunofluorescence/Immunocytochemistry: (1/100 - 1/500). Researchers should empirically determine the suitability of the DGKI dgki for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGK iota, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
