Rabbit DGK-epsilon Polyclonal Antibody | anti-DGK antibody

DGK-epsilon (S194) Polyclonal Antibody

Cat. Num. AAA3002403

• Gene Names: DGKE; DGK; AHUS7; DAGK5; DAGK6; NPHS7
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DGK-epsilon; Polyclonal Antibody; DGK-epsilon (S194) Polyclonal Antibody; Diacylglycerol kinase epsilon; DAG kinase epsilon; Diglyceride kinase epsilon; DGKE; DAGK5; anti-DGK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DGK-epsilon (S194) polyclonal antibody detects endogenous levels of DGK-epsilon protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 567

• Applicable Applications for anti-DGK antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 180-230 of Human DGK-epsilon.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of DGK-epsilon (S194) pAb in paraffin-embedded human rectum carcinoma tissue at 1:50.showing cytoplasmic staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-DGK antibody

Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-alpha (80 kDa), -beta (90 kDa), and -gamma (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-delta (130 kDa) and DGK-i (130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK-epsilon (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK-zeta (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-theta (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.

NCBI and Uniprot Product Information

• NCBI GI #: 4503313
• NCBI GeneID: 8526
• NCBI Accession #: NP_003638.1
• NCBI GenBank Nucleotide #: NP_003638.1
• UniProt Accession #: P52429
• Molecular Weight: ~ 64kDa
• NCBI Official Full Name: diacylglycerol kinase epsilon
• NCBI Official Synonym Full Names: diacylglycerol kinase epsilon
• NCBI Official Symbol: DGKE
• NCBI Official Synonym Symbols: DGK; AHUS7; DAGK5; DAGK6; NPHS7
• NCBI Protein Information: diacylglycerol kinase epsilon
• UniProt Protein Name: Diacylglycerol kinase epsilon
• Protein Family: Deoxyguanosine kinase
• UniProt Gene Name: DGKE
• UniProt Synonym Gene Names: DAGK5; DAG kinase epsilon; DGK-epsilon
• UniProt Entry Name: DGKE_HUMAN

NCBI Description

Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKE: Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition. Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: Lipid Metabolism - glycerolipid; Lipid Metabolism - glycerophospholipid; Membrane protein, multi-pass; Membrane protein, integral; EC 2.7.1.107; Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: metal ion binding; diacylglycerol kinase activity; ATP binding; NAD+ kinase activity

Biological Process: platelet activation; protein kinase C activation; blood coagulation; phosphorylation; phospholipid biosynthetic process

Disease: Nephrotic Syndrome, Type 7