Rabbit anti-Human DGCR8 Polyclonal Antibody | anti-DGCR8 antibody

Anti-DGCR8 Antibody, Rabbit Polyclonal

Cat. Num. AAA8109460

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: DGCR8; Polyclonal Antibody; Anti-DGCR8 Antibody; Rabbit Polyclonal; DGCR8 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-C22orf12 Antibody; Anti-DGCRK6 Antibody; Anti-Gy1 Antibody; Anti-pasha Antibody; anti-DGCR8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human DGCR8
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-DGCR8 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:50-1:200; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human DGCR8 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human DGCR8 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-DGCR8 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:86 kDa (We are unsure as to the identity of these extra bands.))

Immunohistochemistry (IHC)

(Immunochemical staining of human DGCR8 in human duodenum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of DGCR8 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human DGCR8 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.)

Related Product Information for anti-DGCR8 antibody

DiGeorge syndrome chromosomal region 8 (DGCR8), a double-stranded-RNA-binding protein, participates in the miRNA biogenesis pathway and contributes to miRNA maturation by interacting with the RNAase III enzyme Drosha in cell nuclei. DGCR8, which is known as Pasha in flies and worms, is a 773-amino-acid (aa) protein critical for microRNA (miRNA) biogenesis via the formation of a microprocessor complex with Drosha. This complex processes primary miRNA in the nucleus. DGCR8 deletion in murine B-cell progenitors impairs the transition from pro-to early pre-B cell stage, recapitulating the phenotypes observed in miR17-92 cluster knockout mice. The DGCR8-mediated miRNA pathway is required for maintaining blood pressure, vascular reactivity and vascular wall remodeling at the postnatal stages.

References

Zou Y, et al. (2018) Deletion of dgcr8 in vsmcs of adult mice results in loss of vascular reactivity, reduced blood pressure and neointima formation. Sci Rep 8 (1): 1468.Hoffmann N, et al. (2018) Dgcr8 promotes neural progenitor expansion and represses neurogenesis in the mouse embryonic neocortex. Front Neurosci 12 281.Deng L, et al. (2019) Stabilizing heterochromatin by dgcr8 alleviates senescence and osteoarthritis. Nat Commun 10 (1): 3329.