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Rabbit dgcE Polyclonal Antibody | anti-dgcE antibody

Rabbit anti-Escherichia coli (strain K12) dgcE Polyclonal Antibody

Gene Names
yegE; ECK2061; JW2052
Reactivity
E Coli (strain K12)
Applications
ELISA, Western Blot
Purity
Protein A/G
Synonyms
dgcE; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) dgcE Polyclonal Antibody; dgcE Antibody; Probable diguanylate cyclase YegE; DGC; EC 2.7.7.65; yegE b2067 JW2052; anti-dgcE antibody
Ordering
Host
Rabbit
Reactivity
E Coli (strain K12)
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A/G
Form/Format
Liquid
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-dgcE antibody
ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
Immunogen
Recombinant Escherichia coli (strain K12) dgcE protein
Conjugation
Non-conjugate
Modification
Unmodified
Deliverables
1. 200ug recombinant immunogen protein/peptide (positive control).
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
Quality Guarantee
1. Antibody purity >90% confirmed by SDS-PAGE.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
Complementary Antibody
Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:
* 6*His Monoclonal Antibody #MBS1491670
* DYKDDDDK tag Monoclonal Antibody #MBS1493946
* GAPDH Monoclonal Antibody #MBS1495524
* GFP Monoclonal Antibody #MBS1496050
* Myc tag Monoclonal Antibody #MBS1497694
* GST Monoclonal Antibody #MBS1497871
* Sumo tag Monoclonal Antibody #MBS1498878
* Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510
* HA-Tag Monoclonal Antibody #MBS7135631
* E-Tag Monoclonal Antibody #MBS7135632
* TUBA1A Monoclonal Antibody #MBS7135645
* TUBB Monoclonal Antibody #MBS9018332
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
123,887 Da
NCBI Official Full Name
putative diguanylate cyclase
NCBI Official Symbol
yegE
NCBI Official Synonym Symbols
ECK2061; JW2052
NCBI Protein Information
putative diguanylate cyclase
UniProt Protein Name
Probable diguanylate cyclase DgcE
UniProt Gene Name
dgcE
UniProt Synonym Gene Names
DGCCurated

NCBI Description

A yegE null mutation partially suppresses the motility defect of a yhjH mutation; yegE partial suppression is best at 28C, full yhjH suppression requires yegE and yedQ mutations; a yeaJ null mutation is a better yhjH suppressor at 37C; full yhjH suppression requires yegE, yedQ and yeaJ null mutations when grown at 37C (Pesavento, 2008). [More information is available at EcoGene: EG12396]. YegE is a GGDEF/EAL protein that is involved in regulation of the switch from flagellar motility to sessile behavior and curli expression . [More information is available at EcoCyc: EG12396].

Uniprot Description

Catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules (). Involved in the control of the switch from cell motility to adhesion via regulation of cellular levels of c-di-GMP (Probable). Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two c-di-GMP control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA and expression of the master biofilm regulator csgD (PubMed:23708798).

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Product Notes

The dgcE dgce (Catalog #AAA7157351) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Escherichia coli (strain K12) dgcE Polyclonal Antibody reacts with E Coli (strain K12) and may cross-react with other species as described in the data sheet. AAA Biotech's dgcE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the dgcE dgce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "dgcE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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