Western Blot (WB)
((0.5ug/ml) staining of Human Liver lysate in the third lane (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat DGAT2 Polyclonal Antibody | anti-DGAT2 antibody
Goat anti-DGAT2 Antibody
Immunohistochemistry: In paraffin embedded Human Liver shows granular cytoplasm staining of hepatocytes. Recommended concentration, 3ug/ml.
Western Blot: In transfected HEK293 transiently expressing DGAT2 bands of approx. 60kDa and 45kDa are observed. These bands are not observed in the non-transfected HEK293. Similar results are observed in Human Liver lysates. The calculated molecular size is 43.8kDa according to NP_115953.2. Recommended concentration, 0.3-1ug/ml.
Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant DGAT2 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1ug/ml.
Western Blot (WB)
((0.5ug/ml) staining of Human Liver lysate in the third lane (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Human Liver lysate in the third lane (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing DGAT2 (lane A) and probed (mock transfection in lane B).)
Western Blot (WB)
(HEK293 overexpressing DGAT2 (lane A) and probed (mock transfection in lane B).)
Immunohistochemistry (IHC)
((3ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
Immunohistochemistry (IHC)
((3ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.)
Testing Data
((0.5ug/ml) as the reporter with as the capture rabbit antibody (2.5ug/ml).)
Testing Data
((0.5ug/ml) as the reporter with as the capture rabbit antibody (2.5ug/ml).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Uniprot Description
DGAT2: Essential acyltransferase that catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for synthesis and storage of intracellular triglycerides. Probably plays a central role in cytosolic lipid accumulation. In liver, is primarily responsible for incorporating endogenously synthesized fatty acids into triglycerides. Functions also as an acyl-CoA retinol acyltransferase (ARAT). Belongs to the diacylglycerol acyltransferase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; EC 2.3.1.20; Transferase; EC 2.3.1.76; Lipid Metabolism - glycerolipid; Cofactor and Vitamin Metabolism - retinol; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11q13.5
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to endoplasmic reticulum membrane; integral to membrane; lipid particle; mitochondrion; perinuclear region of cytoplasm
Molecular Function: 2-acylglycerol O-acyltransferase activity; diacylglycerol O-acyltransferase activity; protein homodimerization activity; retinol O-fatty-acyltransferase activity
Biological Process: cholesterol homeostasis; diacylglycerol metabolic process; fatty acid homeostasis; glycerol metabolic process; negative regulation of fatty acid oxidation; positive regulation of gluconeogenesis; regulation of lipoprotein metabolic process; retinol metabolic process; sequestering of lipid; triacylglycerol biosynthetic process
Research Articles on DGAT2
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Product Notes
The DGAT2 dgat2 (Catalog #AAA420006) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DGAT2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's DGAT2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB), ELISA (EIA). Peptide ELISA: Antibody detection limit dilution 1: 128000. Immunohistochemistry: In paraffin embedded Human Liver shows granular cytoplasm staining of hepatocytes. Recommended concentration, 3ug/ml. Western Blot: In transfected HEK293 transiently expressing DGAT2 bands of approx. 60kDa and 45kDa are observed. These bands are not observed in the non-transfected HEK293. Similar results are observed in Human Liver lysates. The calculated molecular size is 43.8kDa according to NP_115953.2. Recommended concentration, 0.3-1ug/ml. Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant DGAT2 captured by a rabbit antibody. Recommended reporter concentration: 0.5-1ug/ml. Researchers should empirically determine the suitability of the DGAT2 dgat2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: KLEHPTQQDI DLYH. It is sometimes possible for the material contained within the vial of "DGAT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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