Rabbit anti-Human DFFB Polyclonal Antibody | anti-DFFB antibody

DFFB, NT (DFFB, CAD, DFF2, DFF40, DNA fragmentation factor subunit beta, Caspase-activated deoxyribonuclease, Caspase-activated nuclease, DNA fragmentation factor 40kD subunit) (FITC)

Cat. Num. AAA6291819

• Gene Names: DFFB; CAD; CPAN; DFF2; DFF40; DFF-40
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DFFB; Polyclonal Antibody; NT (DFFB; CAD; DFF2; DFF40; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; Caspase-activated nuclease; DNA fragmentation factor 40kD subunit) (FITC); DNA fragmentation factor 40kD subunit; anti-DFFB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DFFB antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DFFB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-terminal region of human DFFB.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DFFB Antibody (N-term) in 293 cell line lysates (35ug/lane). DFFB (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(DFFB Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DFFB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DFFB Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DFFB antibody

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Product Categories/Family for anti-DFFB antibody

Antibodies; Apoptosis; Caspase

References

Hanus, J., et al. Apoptosis 13(3):377-382(2008). Kalinowska-Herok, M., et al. Acta Biochim. Pol. 55(1):21-26(2008). Neimanis, S., et al. J. Biol. Chem. 282(49):35821-35830(2007). Hristoskova, S., et al. J. Cell. Physiol. 213(2):490-494(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 37962942
• NCBI GeneID: 1677
• UniProt Accession #: O76075; O60521; Q5SR22; Q9BYI4; Q9BYI5; Q9BYI6
• Molecular Weight: 39,110 Da
• NCBI Official Full Name: c-truncated caspase-activated DNase
• NCBI Official Synonym Full Names: DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
• NCBI Official Symbol: DFFB
• NCBI Official Synonym Symbols: CAD; CPAN; DFF2; DFF40; DFF-40
• NCBI Protein Information: DNA fragmentation factor subunit beta; caspase-activated DNase; caspase-activated nuclease; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit
• UniProt Protein Name: DNA fragmentation factor subunit beta
• Protein Family: DNA fragmentation factor
• UniProt Gene Name: DFFB
• UniProt Synonym Gene Names: CAD; DFF2; DFF40; CAD; Caspase-activated DNase; CPAN; DFF-40
• UniProt Entry Name: DFFB_HUMAN

NCBI Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]

Uniprot Description

DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Deoxyribonuclease; EC 3.-.-.-

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; cytosol; nucleus

Molecular Function: enzyme binding; deoxyribonuclease activity

Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation; cell structure disassembly during apoptosis