Rabbit DFF40 / DFFB Polyclonal Antibody | anti-DFF40 antibody

Rabbit Polyclonal to Human DFF40 / DFFB

Cat. Num. AAA240363

• Gene Names: DFFB; CAD; CPAN; DFF2; DFF40; DFF-40
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: DFF40 / DFFB; Polyclonal Antibody; Rabbit Polyclonal to Human DFF40 / DFFB; Anti-DFF40 / DFFB Antibody (Internal) IHC-plus; DFFB; Caspase-activated nuclease; DFF-40; DFF40; Caspase-activated DNase; CPAN; DFF2; Human DFF40; anti-DFF40 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: peptide corresponding to amino acids near the center of murine CAD The sequence differs from human DFF40 by two amino acids (3,4)
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 338

• Applicable Applications for anti-DFF40 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC, IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human DFFB.
• Immunogen Type: Synthetic peptide
• Immunogen: DFF40 / DFFB antibody was raised against synthetic peptide from human DFFB.
• Antigen Modification: Internal
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-DFFB antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 ug/ml.)

Western Blot (WB)

(Western blot of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.)

Related Product Information for anti-DFF40 antibody

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease).

Product Categories/Family for anti-DFF40 antibody

Family: DNase

NCBI and Uniprot Product Information

• NCBI GI #: 4758150
• NCBI GeneID: 1677
• NCBI Accession #: NP_004393.1
• NCBI GenBank Nucleotide #: NM_004402.3
• UniProt Accession #: O76075; O60521; Q5SR22; Q9BYI4; Q9BYI5; Q9BYI6
• Molecular Weight: 11,482 Da
• NCBI Official Full Name: DNA fragmentation factor subunit beta isoform 2
• NCBI Official Synonym Full Names: DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
• NCBI Official Symbol: DFFB
• NCBI Official Synonym Symbols: CAD; CPAN; DFF2; DFF40; DFF-40
• NCBI Protein Information: DNA fragmentation factor subunit beta; caspase-activated DNase; caspase-activated nuclease; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit
• UniProt Protein Name: DNA fragmentation factor subunit beta
• UniProt Gene Name: DFFB
• UniProt Synonym Gene Names: CAD; DFF2; DFF40; CAD; Caspase-activated DNase; CPAN; DFF-40
• UniProt Entry Name: DFFB_HUMAN

NCBI Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]

Uniprot Description

DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Deoxyribonuclease; EC 3.-.-.-; Apoptosis

Chromosomal Location of Human Ortholog: 1p36.3

Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; cytosol; nucleus

Molecular Function: enzyme binding; deoxyribonuclease activity

Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation; cell structure disassembly during apoptosis