Western Blot (WB)
(Western blot analysis of DEPDC1B in K562 cell lysate with DEPDC1B antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Rabbit anti-Human DEPDC1B Polyclonal Antibody | anti-DEPDC1B antibody
DEPDC1B Antibody
Gene Names
DEPDC1B; XTP1; BRCC3
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity chromatography purified via peptide column.
Synonyms
DEPDC1B; Polyclonal Antibody; DEPDC1B Antibody; DEP domain-containing 1B; DEP1B; BRCC3; Breast cancer cell 3; XTP1; anti-DEPDC1B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
DEPDC1B antibody is human specific. At least two isoforms are known to exist; this antibody will only detect the largest isoform. DEPDC1B antibody is predicted to not cross-react with DEPDC1A.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid; Supplied in PBS containing 0.02% sodium azide
Concentration
1 mg/mL (varies by lot)
Sequence Length
529
Applicable Applications for anti-DEPDC1B antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
DEPDC1B antibody can be used for detection of DEPDC1B by Western blot at 1 - 2ug/ml. Antibody can also be used for immunohistochemistry starting at 5 ug/mL.
Optimal dilutions for each application to be determined byt the researcher.
Optimal dilutions for each application to be determined byt the researcher.
Immunogen
DEPDC1B antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DEPDC1B. The imunnogen is located within the last 50 amino acids of DEPDC1B
Positive Control
1. Cat No. 1204 - K562 Cell Lysate
2. Cat. No. 10-901 - Human Spleen Tissue Side
2. Cat. No. 10-901 - Human Spleen Tissue Side
Conjugate
Unconjugated
Homology
Predicted species reactivity based on immunogen sequence: Mouse: (94%), Chicken: (88%)
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of DEPDC1B in K562 cell lysate with DEPDC1B antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of DEPDC1B in K562 cell lysate with DEPDC1B antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of DEPDC1B in human spleen tissue with DEPDC1B antibody at 5 μg/ml.)
Immunohistochemistry (IHC)
(Immunohistochemistry of DEPDC1B in human spleen tissue with DEPDC1B antibody at 5 μg/ml.)
Related Product Information for anti-DEPDC1B antibody
The DEP domain-containing 1B (DEPDC1B) protein was initially identified as a radiation response biomarker in human lymphoblastoid cell lines (1). Like the related protein DEPDC1A, expression of DEPDC1B correlates with a poor prognosis in cancer patients (2,3). Studies have shown that DEPDC1B levels are upregulated in non-small cell lung cancer (NSCLC) and have an inverse correlation with patient survival. Ectopic expression of DEPDC1B in NSCLC activated Wnt/B-catenin signaling and enhanced cell migration and invasion while silencing DEPDC1B expression suppressed these traits (2).
Product Categories/Family for anti-DEPDC1B antibody
References
Niu N, Qin Y, Fridley BL, et al. Radiation pharmacogenomics: a genome-wide association approach to identify radiation response biomarkers using human lymphoblastoid cell lines. Genome Res. 2010; 20:1482-92. Yang Y, Liu L, Cai J, et al. DEPDC1B enhances migration and invasion of non-small cell lung cancer cells via activating Wnt/B-catenin signaling. Biochem. Biophys. Res. Commun. 2014; 450:899-905. Kassambara A, Schoenhals M, Moreaux J, et al. Inhibition of DEPDC1A, a bad prognostic marker in multiple myeloma, delays growth and induces mature plasma cell markers in malignant plasma cells. PLoS One 2013; 8:e62752.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 58 kDa
Observed: 57 kDa
Observed: 57 kDa
NCBI Official Full Name
DEP domain-containing protein 1B isoform 1
NCBI Official Synonym Full Names
DEP domain containing 1B
NCBI Official Symbol
DEPDC1B
NCBI Official Synonym Symbols
XTP1; BRCC3
NCBI Protein Information
DEP domain-containing protein 1B
UniProt Protein Name
DEP domain-containing protein 1B
Protein Family
UniProt Gene Name
DEPDC1B
UniProt Synonym Gene Names
XTP8
UniProt Entry Name
DEP1B_HUMAN
Uniprot Description
DEPDC1B: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: GAPs, Rac/Rho; GAPs
Chromosomal Location of Human Ortholog: 5q12.1
Cellular Component: cytosol
Molecular Function: GTPase activator activity
Biological Process: cell migration; positive regulation of GTPase activity; positive regulation of Wnt receptor signaling pathway; regulation of small GTPase mediated signal transduction
Research Articles on DEPDC1B
Similar Products
Product Notes
The DEPDC1B depdc1b (Catalog #AAA154376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DEPDC1B Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DEPDC1B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin. DEPDC1B antibody can be used for detection of DEPDC1B by Western blot at 1 - 2ug/ml. Antibody can also be used for immunohistochemistry starting at 5 ug/mL. Optimal dilutions for each application to be determined byt the researcher. Researchers should empirically determine the suitability of the DEPDC1B depdc1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEPDC1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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