Rabbit anti-Human, Mouse DENND1A Polyclonal Antibody | anti-DENND1A antibody
DENND1A Antibody
Gene Names
DENND1A; FAM31A; KIAA1608
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
DENND1A; Polyclonal Antibody; DENND1A Antibody; Connecdenn 1; Connecdenn; DEN1A_HUMAN; DENN domain-containing protein 1A; DENN/MADD domain containing 1A; dennd1a; FAM31A; FLJ21129; FLJ38464; KIAA1608; OTTHUMP00000022090; Protein FAM31A; RP11 230L22.3; anti-DENND1A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
DENND1A Antibody detects endogenous levels of DENND1A.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-DENND1A antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human DENND1A.
Conjugation
Unconjugated
Post Translational Modifications
Phosphorylated on serine and/or threonine in an Akt-dependent manner. Phosphorylation probably regulates the guanine nucleotide exchange factor (GEF) activity, possibly by disrupting an intramolecular interaction between the DENN domain and the C-terminus of the protein, thereby relieving the autoinhibition.
Subunit Structure
Interacts with RAB35. Interacts with clathrin and with the adapter protein complex 2, AP-2. Interacts with ITSN1 AND SH3GL2 (By similarity). Interacts (when phosphorylated) with YWHAE.
Function
Guanine nucleotide exchange factor (GEF) regulating clathrin-mediated endocytosis through RAB35 activation. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB35 into its active GTP-bound form. Regulates clathrin-mediated endocytosis of synaptic vesicles and mediates exit from early endosomes. Binds phosphatidylinositol-phosphates (PtdInsPs), with some preference for PtdIns(3)P (By similarity).
Note
Associates to membranes via lipid-binding activity.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Product Categories/Family for anti-DENND1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
110,577 Da
NCBI Official Full Name
DENN domain-containing protein 1A isoform 1
NCBI Official Synonym Full Names
DENN/MADD domain containing 1A
NCBI Official Symbol
DENND1A
NCBI Official Synonym Symbols
FAM31A; KIAA1608
NCBI Protein Information
DENN domain-containing protein 1A; connecdenn 1
UniProt Protein Name
DENN domain-containing protein 1A
Protein Family
UniProt Gene Name
DENND1A
UniProt Synonym Gene Names
FAM31A; KIAA1608; Connecdenn
UniProt Entry Name
DEN1A_HUMAN
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Product Notes
The DENND1A dennd1a (Catalog #AAA9630213) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DENND1A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DENND1A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the DENND1A dennd1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DENND1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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