Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Western Blot: Sample: Recombinant DEFb1, Human.)

Rabbit Defensin Beta 1 Polyclonal Antibody | anti-DEFb1 antibody

Polyclonal Antibody to Defensin Beta 1 (DEFb1)

Gene Names
DEFB1; BD1; HBD1; DEFB-1; DEFB101
Reactivity
Homo sapiens (Human)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Defensin Beta 1; Polyclonal Antibody; Polyclonal Antibody to Defensin Beta 1 (DEFb1); anti-DEFb1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Homo sapiens (Human)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.31mg/ml (varies by lot)
Sequence Length
68
Applicable Applications for anti-DEFb1 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
WB: 0.5-2ug/mL
IHC: 5-20ug/mL
ICC: 5-20ug/mL
Optimal working dilutions must be determined by end user
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Pig
Immunogen
Recombinant DEFb1 (Gly22~Lys68) expressed in E.coli (MBS2031273).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant DEFb1, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant DEFb1, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples:Human Kidney Tissue; Primary Ab:20 ug/ml Rabbit Anti-Human DEFb1Antibody Second Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgGPolyclonal Antibody (Catalog: MBS2086047))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples:Human Kidney Tissue; Primary Ab:20 ug/ml Rabbit Anti-Human DEFb1Antibody Second Ab: 2ug/ml HRP-Linked Caprine Anti-Rabbit IgGPolyclonal Antibody (Catalog: MBS2086047))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
beta-defensin 1 preproprotein
NCBI Official Synonym Full Names
defensin beta 1
NCBI Official Symbol
DEFB1
NCBI Official Synonym Symbols
BD1; HBD1; DEFB-1; DEFB101
NCBI Protein Information
beta-defensin 1
UniProt Protein Name
Beta-defensin 1
Protein Family
UniProt Gene Name
DEFB1
UniProt Synonym Gene Names
BD1; HBD1; BD-1; hBD-1

NCBI Description

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in the resistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin family member, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. [provided by RefSeq, Jul 2008]

Uniprot Description

defensin, beta 1: Has bactericidal activity. Belongs to the beta-defensin family.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular region; extracellular space; extrinsic component of membrane; Golgi lumen

Molecular Function: CCR6 chemokine receptor binding; identical protein binding; protein binding

Biological Process: acute inflammatory response; antibacterial humoral response; antimicrobial humoral response; cAMP-mediated signaling; chemotaxis; defense response to bacterium; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; G-protein coupled receptor signaling pathway; immune response; innate immune response; innate immune response in mucosa; response to bacterium; response to testosterone stimulus

Research Articles on DEFb1

Similar Products

Product Notes

The DEFb1 defb1 (Catalog #AAA2027009) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Defensin Beta 1 (DEFb1) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Defensin Beta 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). WB: 0.5-2ug/mL IHC: 5-20ug/mL ICC: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the DEFb1 defb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Defensin Beta 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.