Rabbit anti-Human, Mouse Defender of Apoptotic Cell Death 1 Polyclonal Antibody | anti-DAD1 antibody

Defender of Apoptotic Cell Death 1 (DAD1)

Cat. Num. AAA618933

• Gene Names: DAD1; DAD1
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Defender of Apoptotic Cell Death 1; Polyclonal Antibody; Defender of Apoptotic Cell Death 1 (DAD1); Anti -Defender of Apoptotic Cell Death 1 (DAD1); anti-DAD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Specific for defender of apoptotic cell death 1 (DAD1).
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

• Applicable Applications for anti-DAD1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot..; Dilution: Western Blot: 0.5-2.0 ug/ml, detects a band of approximately 22kD in HepG2 cell lysates.; Optimal dilution determined by the researcher.
• Immunogen: Synthetic peptide corresponding to a fourteen amino acid sequence within the C-Terminal region of human DAD1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DAD1 antibody

DAD1 was discovered as a negative regulator of programmed cell death, and has since been identified as a subunit of the mammalian oligosaccharyltransferase complex (OCT). DAD1 is required for the function and structural integrity of OCT.

Product Categories/Family for anti-DAD1 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 55926192
• NCBI GeneID: 395343
• NCBI Accession #: NP_001007474.1
• NCBI GenBank Nucleotide #: NM_001007473.1
• UniProt Accession #: O13113; Q9I898
• Molecular Weight: 12,926 Da
• NCBI Official Full Name: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
• NCBI Official Synonym Full Names: defender against cell death 1
• NCBI Official Symbol: DAD1
• NCBI Official Synonym Symbols: DAD1
• NCBI Protein Information: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DAD-1; apoptotic suppressor; defender against death protein 1; defender against cell death 1 protein; oligosaccharyl transferase subunit DAD1
• UniProt Protein Name: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
• Protein Family: DASH complex
• UniProt Gene Name: DAD1
• UniProt Entry Name: DAD1_CHICK

Uniprot Description

Function: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis

Catalytic activity: Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.

Subunit structure: Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes

Subcellular location: Membrane; Multi-pass membrane protein

Potential.

Sequence similarities: Belongs to the DAD/OST2 family.