HSP60 recombinant protein

HSP60 recombinant protein

Cat. Num. AAA515419

• Gene Names: HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• Applications: Western Blot
• Synonyms: HSP60; HSP60 recombinant protein; HSPD1; CPN60; GROEL; HSP65; SPG13; HuCHA60

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 2339

• Applicable Applications for HSP60 recombinant protein: Kinase Assay, Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant full-length human HSP60 was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for HSP60 recombinant protein

Recombinant full-length human HSP60 was expressed in E. coli cells using a N-terminal GST tag.

Scientific Background: HSP60 is a chaperonin protein, required for ATP-dependent folding of precursor polypeptides and complex assembly and prevents aggregation and mediates protein refolding after heat shock. Mutations and variations in the HSP 60 gene product may be implicated in genetic diseases (1). HSP60 has been strongly implicated as an example of molecular mimicry in the pathogenicity of autoimmune diseases in T cell-mediated protection. Human HSP60 was found to activate the complement system in normal human serum in a dose-dependent manner. Since complement activation plays an important role in the development of atherosclerosis, the levels of complement-activating anti-HSP60 antibodies are elevated in atherosclerosis-related diseases. (2)

Product Categories/Family for HSP60 recombinant protein

Cell Stress & Chaperone Proteins; Signaling Proteins - Cell Stress & Chaperone Proteins

References

1. Pochon, N A. et al: Genetic complexity of the human hsp 60 gene. Int. Immunol. 1996; 8(2):221-30
2. Zolta¡n, P. et al: Antibodies against human heat-shock protein (hsp) 60 and mycobacterial hsp65 differ in their antigen specificity and complement-activating abililty. Int. Immunol. 1999;11 (9), 1363-1370.

NCBI and Uniprot Product Information

• NCBI GI #: 41399283
• NCBI GeneID: 3329
• NCBI Accession #: NP_002147.2
• NCBI GenBank Nucleotide #: NM_002156
• UniProt Accession #: P10809; Q38L19; Q9UCR6; B2R5M6
• Molecular Weight: ~85 kDa
• NCBI Official Full Name: Homo sapiens heat shock 60kDa protein 1 (chaperonin) (HSPD1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: heat shock 60kDa protein 1 (chaperonin)
• NCBI Official Symbol: HSPD1
• NCBI Official Synonym Symbols: HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
• NCBI Protein Information: 60 kDa heat shock protein, mitochondrial; chaperonin 60; 60 kDa chaperonin; heat shock protein 65; P60 lymphocyte protein; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1
• UniProt Protein Name: 60 kDa heat shock protein, mitochondrial
• Protein Family: Hydrogenosomal chaperonin
• UniProt Gene Name: HSPD1
• UniProt Synonym Gene Names: HSP60; CPN60; HSP-60; Hsp60
• UniProt Entry Name: CH60_HUMAN

NCBI Description

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]

Uniprot Description

Function: Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.

Subunit structure: Interacts with HRAS

By similarity. Interacts with HBV protein X and HTLV-1 protein p40tax. Interacts with ATAD3A. Interacts with METTL20 and METTL21B. Ref.9 Ref.19 Ref.29 Ref.30

Subcellular location: Mitochondrion matrix.

Involvement in disease: Spastic paraplegia 13, autosomal dominant (SPG13) [MIM:605280]: A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.31Leukodystrophy, hypomyelinating, 4 (HLD4) [MIM:612233]: A severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurs within the first two decades of life.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.32

Sequence similarities: Belongs to the chaperonin (HSP60) family.