Rabbit anti-Human DEF6 Polyclonal Antibody | anti-DEF6 antibody

DEF6, CT (DEF6, IBP, Differentially expressed in FDCP 6 homolog, IRF4-binding protein) (FITC)

Cat. Num. AAA6291599

• Gene Names: DEF6; IBP; SLAT; SWAP70L
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DEF6; Polyclonal Antibody; CT (DEF6; IBP; Differentially expressed in FDCP 6 homolog; IRF4-binding protein) (FITC); IRF4-binding protein; anti-DEF6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DEF6 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DEF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 499-528 amino acids from the C-terminal region of human DEF6.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DEF6 Antibody (C-term) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the DEF6 antibody detected the DEF6 protein (arrow).)

Related Product Information for anti-DEF6 antibody

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).

Product Categories/Family for anti-DEF6 antibody

Antibodies; Binding Proteins

References

Han, S., et al. Hum. Immunol. 71(7):727-730(2010). Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010). Zhao, J., et al. BMC Med. Genet. 11, 96 (2010): Zhang, Z., et al. Cancer Epidemiol 33(2):130-136(2009). Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 145312241
• NCBI GeneID: 50619
• NCBI Accession #: NP_071330.3
• NCBI GenBank Nucleotide #: NM_022047.3
• UniProt Accession #: Q9H4E7; Q86VF4
• Molecular Weight: 73,910 Da
• NCBI Official Full Name: differentially expressed in FDCP 6 homolog
• NCBI Official Synonym Full Names: DEF6, guanine nucleotide exchange factor
• NCBI Official Symbol: DEF6
• NCBI Official Synonym Symbols: IBP; SLAT; SWAP70L
• NCBI Protein Information: differentially expressed in FDCP 6 homolog
• UniProt Protein Name: Differentially expressed in FDCP 6 homolog
• Protein Family: Differentially expressed in FDCP
• UniProt Gene Name: DEF6
• UniProt Synonym Gene Names: IBP; DEF-6
• UniProt Entry Name: DEFI6_HUMAN

NCBI Description

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).[supplied by OMIM, Mar 2008]

Uniprot Description

DEF6: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42. Can regulate cell morphology in cooperation with activated RAC1. Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling.

Protein type: GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 6p21.33-p21.1

Cellular Component: membrane; cytoplasm; plasma membrane; intercellular junction; nucleus