Western Blot (WB)
((0.3ug/ml) staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat DEAD-box protein 6 Polyclonal Antibody | anti-DDX6 antibody
Goat anti-DEAD-box protein 6 Antibody
Western Blot: Approx 50kDa band observed in lysates of cell lines Daudi, Jurkat and HepG2 (calculated MW of 53.2kDa according to NP_004388.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Daudi (A), Jurkat (B) and HepG2 (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012]
Uniprot Description
DDX6: an oncogenic ATP-dependent helicase of the DEAD box family. Interacts with argonaute proteins, Ago1 and Ago2, and is a general repressor of translation in human cells by miRNA-induced gene silencing. In the process of mRNA degradation, may play a role in mRNA decapping.
Protein type: Oncoprotein; RNA-binding; Helicase; RNA processing; EC 3.6.4.13
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: cytoplasm; cytosol; intracellular membrane-bound organelle; membrane; stress granule
Molecular Function: ATP binding; ATP-dependent RNA helicase activity; helicase activity; protein binding; protein domain specific binding; RNA helicase activity
Biological Process: cytoplasmic mRNA processing body assembly; negative regulation of neuron differentiation; regulation of translation; RNA secondary structure unwinding; stem cell maintenance; viral RNA genome packaging
Research Articles on DDX6
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Product Notes
The DDX6 ddx6 (Catalog #AAA420268) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DEAD-box protein 6 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's DEAD-box protein 6 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 50kDa band observed in lysates of cell lines Daudi, Jurkat and HepG2 (calculated MW of 53.2kDa according to NP_004388.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the DDX6 ddx6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: AEYHSEPVED EKP. It is sometimes possible for the material contained within the vial of "DEAD-box protein 6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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