Rabbit DDX6 Polyclonal Antibody | anti-DDX6 antibody

DDX6 Polyclonal Antibody

Cat. Num. AAA3012586

• Gene Names: DDX6; P54; RCK; HLR2; IDDILF
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: ProA affinity purified
• Synonyms: DDX6; Polyclonal Antibody; DDX6 Polyclonal Antibody; HLR2; RCK; ATP dependent RNA helicase DDX6; ATP dependent RNA helicase p54; DEAD (Asp Glu Ala Asp) box polypeptide 6; DEAD box 6; DEAD box protein 6; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 6 (RNA helicase 54kD); FLJ36338; Oncogene RCK; P54; Probable ATP-dependent RNA helicase DDX6; anti-DDX6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DDX6 polyclonal antibody detects endogenous levels of DDX6 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 483

• Applicable Applications for anti-DDX6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1,000; IHC: 1:50-1:100
• Immunogen: Recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of DDX6 on different lysates using anti-DDX6 antibody at 1/500 dilution. Positive control:Lane 1: Human colon Lane 2: SH-SY-5YLane 3: SiHa Lane 4: K562)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-DDX6 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-DDX6 antibody

RCK, also known as DDX6 and P54, is a member of the DEAD-box RNA helicase family of proteins, all of which share common protein motifs. Found in most tissues, RCK is an unwindase that exhibits ATP-dependent RNA un-winding activity, as well as the ability to decay RNA in the 5'-3' direction. In non-malignant cells, RCK is associated with all processes of normal RNA metabolism including splicing, export and translation initiation. Mutations in the gene encoding RCK can cause the protein to be overexpressed, changing its function to that of an oncogene that positively regulates the expression of genes involved in cell growth and proliferation. It is believed that, through its unwindase activity, the main function of RCK is to downregulate mRNA expression and maintain normal transcriptional levels within the cell.

NCBI and Uniprot Product Information

• NCBI GI #: 116241327
• NCBI GeneID: 1656
• UniProt Accession #: P26196; P54823
• Molecular Weight: 54kDa
• NCBI Official Full Name: Probable ATP-dependent RNA helicase DDX6
• NCBI Official Synonym Full Names: DEAD-box helicase 6
• NCBI Official Symbol: DDX6
• NCBI Official Synonym Symbols: P54; RCK; HLR2; IDDILF
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX6
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX6
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DDX6
• UniProt Synonym Gene Names: HLR2; RCK
• UniProt Entry Name: DDX6_HUMAN

NCBI Description

This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Mar 2012]

Uniprot Description

DDX6: an oncogenic ATP-dependent helicase of the DEAD box family. Interacts with argonaute proteins, Ago1 and Ago2, and is a general repressor of translation in human cells by miRNA-induced gene silencing. In the process of mRNA degradation, may play a role in mRNA decapping.

Protein type: RNA processing; Oncoprotein; EC 3.6.4.13; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: intracellular membrane-bound organelle; membrane; stress granule; cytoplasm; cytosol

Molecular Function: protein domain specific binding; protein binding; helicase activity; ATP binding; RNA helicase activity

Biological Process: gene expression; cytoplasmic mRNA processing body assembly; mRNA catabolic process, deadenylation-dependent decay