Rabbit anti-Human DDX5 Polyclonal Antibody | anti-DDX5 antibody

DDX5, CT (Probable ATP-dependent RNA Helicase DDX5, DEAD Box Protein 5, RNA Helicase p68, HLR1, HELR, G17P1, DDX5) (MaxLight 650)

Cat. Num. AAA6472127

• Gene Names: DDX5; p68; HLR1; G17P1; HUMP68
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: DDX5; Polyclonal Antibody; CT (Probable ATP-dependent RNA Helicase DDX5; DEAD Box Protein 5; RNA Helicase p68; HLR1; HELR; G17P1; DDX5) (MaxLight 650); anti-DDX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDX5.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-DDX5 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human DDX5 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of DDX5 antibody (C-term) in T47D cell line lysates (35ug/lane). DDX5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX5 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-DDX5 antibody

Antibodies; DEAD Box Proteins

References

1. Ong S.E., Mittler G.Nat. Methods 1:119-126(2004). 2. Daub H., Olsen J.V.Mol. Cell 31:438-448(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4758138
• NCBI GeneID: 1655
• NCBI Accession #: NP_004387
• NCBI GenBank Nucleotide #: NM_004396
• UniProt Accession #: P17844; O75681; Q53Y61; B4DLW8; B5BU21; D3DU32; E7ETL9
• Molecular Weight: 60,563 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX5
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box helicase 5
• NCBI Official Symbol: DDX5
• NCBI Official Synonym Symbols: p68; HLR1; G17P1; HUMP68
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD box protein 5; DEAD box-5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); RNA helicase p68
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX5
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX5
• UniProt Synonym Gene Names: G17P1; HELR; HLR1
• UniProt Entry Name: DDX5_HUMAN

Uniprot Description

DDX5: a putative ATP-dependent RNA helicase. A proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Belongs to the DEAD box helicase family. DDX5/DDX17 subfamily. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly.

Protein type: RNA splicing; Spliceosome; Nuclear receptor co-regulator; Helicase; EC 3.6.4.13; RNA-binding; Nucleolus

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; membrane; nucleolus; ribonucleoprotein complex; nucleus

Molecular Function: calmodulin binding; protein binding; enzyme binding; androgen receptor binding; transcription coactivator activity; estrogen receptor binding; calcium-dependent protein binding; RNA helicase activity; ATP-dependent RNA helicase activity; ATP binding

Biological Process: circadian rhythm; nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; regulation of osteoblast differentiation; in utero embryonic development; positive regulation of estrogen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of transcription from RNA polymerase II promoter; cell growth; regulation of viral genome replication; regulation of alternative nuclear mRNA splicing, via spliceosome