Rabbit anti-Human DDX27 Polyclonal Antibody | anti-DDX27 antibody

DDX27, CT (DDX27, RHLP, Probable ATP-dependent RNA helicase DDX27, DEAD box protein 27) (APC)

Cat. Num. AAA6291421

• Gene Names: DDX27; DRS1; RHLP; Drs1p; PP3241; HSPC259; dJ686N3.1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DDX27; Polyclonal Antibody; CT (DDX27; RHLP; Probable ATP-dependent RNA helicase DDX27; DEAD box protein 27) (APC); DEAD box protein 27; anti-DDX27 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DDX27 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DDX27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 777-796 amino acids from the C-terminal region of human DDX27.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DDX27 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DDX27 antibody detected the DDX27 protein (arrow).)

Immunohistochemistry (IHC)

(DDX27 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prsotate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DDX27 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DDX27 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DDX27 antibody

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined. [provided by RefSeq].

Product Categories/Family for anti-DDX27 antibody

Antibodies; DEAD Box Proteins

References

Deloukas, P., et al. Nature 414(6866):865-871(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 224593277
• NCBI GeneID: 55661
• NCBI Accession #: NP_060365.7
• NCBI GenBank Nucleotide #: NM_017895
• UniProt Accession #: Q96GQ7; Q5VXM7; Q8WYG4; Q969N7; Q96F57; Q96L97; Q9BWY9; Q9BXF0; Q9H990; Q9NWU3; A0AVB6; B7ZLY1
• Molecular Weight: 89,835 Da
• NCBI Official Full Name: Homo sapiens DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27), mRNA
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
• NCBI Official Symbol: DDX27
• NCBI Official Synonym Symbols: DRS1; RHLP; Drs1p; PP3241; HSPC259; dJ686N3.1
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX27; DEAD box protein 27; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 27; Deficiency of ribosomal subunits protein 1 homolog; RNA helicase-like protein
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX27
• Protein Family: Probable ATP-dependent RNA helicase
• UniProt Gene Name: DDX27

NCBI Description

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

DDX27: Probable ATP-dependent RNA helicase. Belongs to the DEAD box helicase family. DDX27/DRS1 subfamily.

Protein type: EC 3.6.4.13; Helicase; RNA-binding

Chromosomal Location of Human Ortholog: 20q13.13

Cellular Component: chromosome; cytoplasm; nucleolus

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; protein binding; RNA binding

Biological Process: RNA secondary structure unwinding; rRNA processing