Rabbit DDX1 Polyclonal Antibody | anti-DDX1 antibody

DDX1 Antibody

Cat. Num. AAA9629814

• Gene Names: DDX1; DBP-RB; UKVH5d
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DDX1; Polyclonal Antibody; DDX1 Antibody; ATP dependent helicase DDX1; ATP-dependent RNA helicase DDX1; DBP RB; DBP-RB; DDX1_HUMAN; DEAD (Asp Glu Ala Asp) box polypeptide 1; DEAD box 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 1; anti-DDX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: DDX1 Antibody detects endogenous levels of DDX1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-DDX1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human DDX1.
• Conjugation: Unconjugated
• Similarity: The helicase domain is involved in the stimulation of RELA transcriptional activity. Belongs to the DEAD box helicase family. DDX1 subfamily.
• Post Translational Modifications: Phosphorylated by ATM kinase; phosphorylation is increased in response to ionizing radiation (IR).
• Subunit Structure: (Microbial infection) Interacts with Venezuelan equine encephalitis virus non-structural protein 3.Found in a multi-helicase-TICAM1 complex at least composed of DHX36, DDX1, DDX21 and TICAM1; this complex exists in resting cells with or without poly(I:C) RNA ligand stimulation. Interacts with DHX36. Interacts (via B30.2/SPRY domain) with DDX21 (via N-terminus); this interaction serves as bridges to TICAM1 (By similarity). Interacts with FAM98A (via N- and C-terminus). Interacts with MBNL1. Interacts with CSTF2. Interacts with HNRNPK. Interacts with ATM. Interacts with RELA (via C-terminus). Component of the tRNA-splicing ligase complex. Interacts with PQBP1. Interacts with PHF5A (via C-terminus) (By similarity). Interacts with ERCC6.(Microbial infection) Interacts with Rev of HIV-1.(Microbial infection) Interacts with Severe acute respiratory syndrome coronavirus (SARS-CoV) (via N-terminus). Interacts (via C-terminus) with the replicase polyprotein 1ab Nsp14 of the Avian infectious bronchitis virus (IBV).
• Tissue Specificity: Highest levels of transcription in 2 retinoblastoma cell lines and in tissues of neuroectodermal origin including the retina, brain, and spinal cord.
• Function: Acts as an ATP-dependent RNA helicase, able to unwind both RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang nuclease activity. Possesses ATPase activity on various RNA, but not DNA polynucleotides. May play a role in RNA clearance at DNA double-strand breaks (DSBs), thereby facilitating the template-guided repair of transcriptionally active regions of the genome. Together with RELA, acts as a coactivator to enhance NF-kappa-B-mediated transcriptional activation. Acts as a positive transcriptional regulator of cyclin CCND2 expression. Binds to the cyclin CCND2 promoter region. Associates with chromatin at the NF-kappa-B promoter region via association with RELA. Binds to poly(A) RNA. May be involved in 3'-end cleavage and polyadenylation of pre-mRNAs. Component of the tRNA-splicing ligase complex required to facilitate the enzymatic turnover of catalytic subunit RTCB: together with archease (ZBTB8OS), acts by facilitating the guanylylation of RTCB, a key intermediate step in tRNA ligation. Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1. Specifically binds (via helicase ATP-binding domain) on both short and long poly(I:C) dsRNA (By similarity).(Microbial infection) Required for HIV-1 Rev function as well as for HIV-1 and coronavirus IBV replication. Binds to the RRE sequence of HIV-1 mRNAs.(Microbial infection) Required for Coronavirus IBV replication.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-DDX1 antibody

Nucleus; Cytoplasm; Cytoplasmic granule; Cytoplasm; Cytosol; Mitochondrion; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 4826686
• NCBI GeneID: 1653
• NCBI Accession #: NP_004930
• NCBI GenBank Nucleotide #: NM_004939
• UniProt Accession #: Q92499; B4DME8; B4DPN6
• Molecular Weight: 68,712 Da
• NCBI Official Full Name: ATP-dependent RNA helicase DDX1
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box helicase 1
• NCBI Official Symbol: DDX1
• NCBI Official Synonym Symbols: DBP-RB; UKVH5d
• NCBI Protein Information: ATP-dependent RNA helicase DDX1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1
• UniProt Protein Name: ATP-dependent RNA helicase DDX1
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DDX1
• UniProt Synonym Gene Names: DBP-RB
• UniProt Entry Name: DDX1_HUMAN