Immunoprecipitation (IP)
(DDR1 was immunoprecipitated using:Lane A:0.5 mg SW480 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-DDR1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DDR1 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 101 kDaObserved band size: 130 kDa)
Rabbit anti-Human DDR1 Polyclonal Antibody | anti-DDR1 antibody
Anti-DDR1 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 1-2uL/mg of lysate
Immunoprecipitation (IP)
(DDR1 was immunoprecipitated using:Lane A:0.5 mg SW480 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-DDR1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DDR1 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 101 kDaObserved band size: 130 kDa)
Immunoprecipitation (IP)
(DDR1 was immunoprecipitated using:Lane A:0.5 mg SW480 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-DDR1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DDR1 rabbit polyclonal antibody,at 1:1000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 101 kDaObserved band size: 130 kDa)
Western Blot (WB)
(Anti-DDR1 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:101 kDaObserved band size:130 kDa)
Western Blot (WB)
(Anti-DDR1 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:101 kDaObserved band size:130 kDa)
NCBI and Uniprot Product Information
Uniprot Description
DDR1: Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11. Interacts (via PPxY motif) with WWC1 (via WW domains) in a collagen-regulated manner. Forms a tripartite complex with WWC1 and PRKCZ, but predominantly in the absence of collagen. Interacts (tyrosine phosphorylated) with SHC1. Interacts with SRC. Interacts with MYH9. Interacts with CDH1. Interacts with PTPN11. Interacts with NCK2. Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells. Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.10.1; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; DDR family
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; receptor complex
Molecular Function: collagen binding; protein binding; metal ion binding; transmembrane receptor protein tyrosine kinase activity; ATP binding
Biological Process: lactation; negative regulation of cell proliferation; extracellular matrix organization and biogenesis; regulation of cell-matrix adhesion; protein amino acid autophosphorylation; smooth muscle cell migration; ear development; regulation of cell growth; cell adhesion; embryo implantation
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Product Notes
The DDR1 ddr1 (Catalog #AAA8103730) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DDR1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:1000 ELISA: 1:5000-1:10,000 IP: 1-2uL/mg of lysate. Researchers should empirically determine the suitability of the DDR1 ddr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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