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Western Blot (WB) (WB Suggested Anti-DDOST Antibody Titration: 5.0ug/mlPositive Control: Jurkat cell lysate)

Rabbit DDOST Polyclonal Antibody | anti-DDOST antibody

DDOST antibody - N-terminal region

Gene Names
DDOST; OST; WBP1; AGER1; CDG1R; GATD6; OST48; OKSWcl45
Reactivity
Tested: Human
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
Western Blot
Purity
Protein A purified
Synonyms
DDOST; Polyclonal Antibody; DDOST antibody - N-terminal region; anti-DDOST antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Tested: Human
Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Clonality
Polyclonal
Purity/Purification
Protein A purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
Batch dependent within range: 0.5 - 1 mg/ml (varies by lot)
Sequence
Synthetic peptide located within the following region: SPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFD
Sequence Length
456
Applicable Applications for anti-DDOST antibody
Western Blot (WB)
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human DDOST
Protein Interactions
HUWE1; VAPB; UBC; FAF1; STAU1; ASB11; TRIM13; SUZ12; EED; CLN3; NXT1; SRSF11; FN1; ATF2; STT3B; MAGT1; ZMPSTE24; VDAC2; SSR4; SSR3; SLC25A3; ILF2; KRTCAP2; FBXO6; UBD; AMFR; AUP1; TMEM173; UNC93B1; MAPK6; HDAC5; AGO3; BRCC3; GSTK1; TMBIM4; PGRMC1; SAP18
Preparation and Storage
For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(WB Suggested Anti-DDOST Antibody Titration: 5.0ug/mlPositive Control: Jurkat cell lysate)

Western Blot (WB) (WB Suggested Anti-DDOST Antibody Titration: 5.0ug/mlPositive Control: Jurkat cell lysate)
Related Product Information for anti-DDOST antibody
This is a rabbit polyclonal antibody against DDOST. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
Product Categories/Family for anti-DDOST antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46kDa
NCBI Official Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
NCBI Official Synonym Full Names
dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit
NCBI Official Symbol
DDOST
NCBI Official Synonym Symbols
OST; WBP1; AGER1; CDG1R; GATD6; OST48; OKSWcl45
NCBI Protein Information
dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit

NCBI Description

This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]

Research Articles on DDOST

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Product Notes

The DDOST (Catalog #AAA3208254) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDOST antibody - N-terminal region reacts with Tested: Human Predicted: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's DDOST can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DDOST for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: SPSVEDFGGN INVETISAFI DGGGSVLVAA SSDIGDPLRE LGSECGIEFD. It is sometimes possible for the material contained within the vial of "DDOST, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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