Rabbit anti-Human DDIT3 Polyclonal Antibody | anti-DDIT3 antibody

DDIT3 Antibody

Cat. Num. AAA7003677

• Gene Names: DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%,Protein G purified
• Synonyms: DDIT3; Polyclonal Antibody; DDIT3 Antibody; DNA damage-inducible transcript 3 protein (DDIT-3) (C/EBP zeta) (C/EBPhomologous protein) (CHOP) (C/EBP-homologous protein 10) (CHOP-10) (CCAAT/enhancer-binding protein homologous protein) (Growth arrest and DNA damage-inducible protein GADD153); CHOP CHOP10 GADD153; anti-DDIT3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%,Protein G purified
• Form/Format: Liquid
• Sequence Length: 192

• Applicable Applications for anti-DDIT3 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC:1:20-1:200; IF:1:50-1:200
• Immunogen: Recombinant human DNA damage-inducible transcript 3 protein (1-169AA).
• Immunogen Species: Homo sapiens (Human)
• Research Area: Epigenetics and Nuclear Signaling
• Target Names: DDIT3
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-575 / sc-793
• Preparation and Storage: Upon receipt store at -20 degree C or -80 degree C.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human gastric cancer using MBS7003677 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7003677 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7003677 at dilution of 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of PC3 cells using MBS7003677 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

NCBI and Uniprot Product Information

• NCBI GI #: 304282225
• NCBI GeneID: 1649
• NCBI Accession #: NP_001181982.1
• NCBI GenBank Nucleotide #: NM_001195053.1
• UniProt Accession #: P35638; F8VS99
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein isoform 1
• NCBI Official Synonym Full Names: DNA damage inducible transcript 3
• NCBI Official Symbol: DDIT3
• NCBI Official Synonym Symbols: CHOP; CEBPZ; CHOP10; CHOP-10; GADD153
• NCBI Protein Information: DNA damage-inducible transcript 3 protein
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• Protein Family: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: DDIT3
• UniProt Synonym Gene Names: CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10
• UniProt Entry Name: DDIT3_HUMAN

NCBI Description

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

CHOP: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: DNA-binding; Oncoprotein; Transcription factor; Autophagy

Chromosomal Location of Human Ortholog: 12q13.1-q13.2

Cellular Component: cytosol; late endosome; nucleoplasm; nucleus

Molecular Function: cAMP response element binding protein binding; DNA binding; leucine zipper domain binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor activity; transcription factor binding

Biological Process: aging; blood vessel maturation; cell cycle arrest; cell redox homeostasis; cellular protein metabolic process; embryonic organ development; ER overload response; inhibition of CREB transcription factor; mRNA transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; negative regulation of myoblast differentiation; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of interleukin-8 production; positive regulation of neuron apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; proteasomal ubiquitin-dependent protein catabolic process; regulation of transcription in response to stress; regulation of transcription, DNA-dependent; release of sequestered calcium ion into cytosol; response to amphetamine; response to DNA damage stimulus; response to drug; response to hydrogen peroxide; response to nutrient; response to starvation; response to unfolded protein; unfolded protein response; Wnt receptor signaling pathway

Disease: Myxoid Liposarcoma