Rabbit anti-Human, Mouse DDC Polyclonal Antibody | anti-DDC antibody

DDC, NT (DDC, AADC, Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase) (MaxLight 750)

Cat. Num. AAA6291253

• Gene Names: DDC; AADC
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: DDC; Polyclonal Antibody; NT (DDC; AADC; Aromatic-L-amino-acid decarboxylase; DOPA decarboxylase) (MaxLight 750); DOPA decarboxylase; anti-DDC antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DDC. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-DDC antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 32-61 in the N-terminal region of human DDC, UniProt Accession #P20711.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse liver, kidney tissue lysates (15ug/lane) usingMBS6010126. This demonstrates that MBS6010126 detected DDC protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma using MBS6010126 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HepG2 cell using MBS6010126 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HepG2 cells (bottom histogram) using MBS6010126 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DDC antibody

This protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency.

Product Categories/Family for anti-DDC antibody

Antibodies; Enzymes; Decarboxylase

References

1. Kokkinou, I., et al. J. Neuroimmunol. 216 (1-2), 51-58 (2009). 2. Meda, S.A., et al. Neuroimage (2009). Ishikawa, S., et al. J. Biol. Chem. 284(42):28832-28844 (2009). 3. Trevino, L.R., et al. Nat. Genet. 41(9):1001-1005(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503281
• NCBI GeneID: 1644
• NCBI Accession #: NP_000781.1
• NCBI GenBank Nucleotide #: NM_000790.3
• UniProt Accession #: P20711; Q16723; Q5W5T9; Q75MJ6; C9IYA0; E7ER62; E7EU95
• Molecular Weight: 44,239 Da
• NCBI Official Full Name: aromatic-L-amino-acid decarboxylase isoform 1
• NCBI Official Synonym Full Names: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• NCBI Official Symbol: DDC
• NCBI Official Synonym Symbols: AADC
• NCBI Protein Information: aromatic-L-amino-acid decarboxylase
• UniProt Protein Name: Aromatic-L-amino-acid decarboxylase
• Protein Family: Aromatic-L-amino-acid decarboxylase
• UniProt Gene Name: DDC
• UniProt Synonym Gene Names: AADC; AADC; DDC
• UniProt Entry Name: DDC_HUMAN

NCBI Description

The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

DDC: Catalyzes the decarboxylation of L-3,4- dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Amino Acid Metabolism - tryptophan; EC 4.1.1.28; Amino Acid Metabolism - histidine; Amino Acid Metabolism - tyrosine; Nuclear receptor co-regulator; Amino Acid Metabolism - phenylalanine; Lyase

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: synaptic vesicle; cell soma; axon; cytosol

Molecular Function: aromatic-L-amino-acid decarboxylase activity; protein domain specific binding; amino acid binding; protein binding; enzyme binding; pyridoxal phosphate binding

Biological Process: circadian rhythm; amino acid metabolic process; catecholamine biosynthetic process; indolalkylamine biosynthetic process; multicellular organismal aging; isoquinoline alkaloid metabolic process; dopamine biosynthetic process; serotonin biosynthetic process; phytoalexin metabolic process; synaptic vesicle amine transport; response to pyrethroid

Disease: Aromatic L-amino Acid Decarboxylase Deficiency