Rabbit anti-Human DCXR Polyclonal Antibody | anti-DCXR antibody

Anti-DCXR Antibody, Rabbit Polyclonal

Cat. Num. AAA8107403

• Gene Names: DCXR; XR; DCR; HCR2; P34H; HCRII; KIDCR; PNTSU; SDR20C1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: DCXR; Polyclonal Antibody; Anti-DCXR Antibody; Rabbit Polyclonal; DCXR/HCR2 Antibody; Rabbit PAb; Antigen Affinity Purified; dicarbonyl/L-xylulose reductase; Anti-DCR Antibody; Anti-HCR2 Antibody; Anti-HCRII Antibody; Anti-KIDCR Antibody; Anti-P34H Antibody; Anti-PNTSU Antibody; Anti-SDR20C1 Antibody; Anti-XR Antibody; anti-DCXR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human DCXR/HCR2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-DCXR antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:2500-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human DCXR/HCR2 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human DCXR/HCR2 (rh DCXR/HCR2). DCXR/HCR2 specific IgG was purified by Human DCXR/HCR2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human DCXR in human epididymis with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human DCXR in human kidney with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human DCXR in human liver with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).)

Immunoprecipitation (IP)

(DCXR was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg A431 Whole Cell Lysate2 uL anti-DCXR rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DCXR rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 26 kDaObserved band size: 26 kDa)

Western Blot (WB)

(Anti-DCXR rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:27 kDaObserved band size:29 kDa)

Related Product Information for anti-DCXR antibody

DCXR, also known as HCR2, belongs to the short-chain dehydrogenases/reductases (SDR) family. It is highly expressed in kidney, liver and epididymis. In the epididymis, DCXR is mainly expressed in the proximal and distal sections of the corpus region. HCR2 is weakly or not expressed in brain, lung, heart, spleen and testis. DCXR catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. DCXR participates in the uronate cycle of glucose metabolism. It may play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.

References

Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40. Pierce SB, et al. (2011) Garrod's fourth inborn error of metabolism solved by the identification of mutations causing pentosuria. Proc Natl Acad Sci. 108(45):18313-7. Udeshi ND, et al. (2012) Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Mol Cell Proteomics. 11(5):148-59.

NCBI and Uniprot Product Information

• NCBI GI #: 50400451
• NCBI GeneID: 51181
• UniProt Accession #: Q7Z4W1; Q9BTZ3; Q9UHY9
• Molecular Weight: 244
• NCBI Official Full Name: L-xylulose reductase
• NCBI Official Synonym Full Names: dicarbonyl/L-xylulose reductase
• NCBI Official Symbol: DCXR
• NCBI Official Synonym Symbols: XR; DCR; HCR2; P34H; HCRII; KIDCR; PNTSU; SDR20C1
• NCBI Protein Information: L-xylulose reductase; carbonyl reductase 2; carbonyl reductase II; sperm surface protein P34H; kidney dicarbonyl reductase; short chain dehydrogenase/reductase family 20C, member 1
• UniProt Protein Name: L-xylulose reductase
• UniProt Gene Name: DCXR
• UniProt Synonym Gene Names: XR; kiDCR
• UniProt Entry Name: DCXR_HUMAN

NCBI Description

The protein encoded by this gene acts as a homotetramer to catalyze diacetyl reductase and L-xylulose reductase reactions. The encoded protein may play a role in the uronate cycle of glucose metabolism and in the cellular osmoregulation in the proximal renal tubules. Defects in this gene are a cause of pentosuria. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2010]

Uniprot Description

DCXR: Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L- xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules. The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Oxidoreductase; Carbohydrate Metabolism - pentose and glucuronate interconversions; EC 1.1.1.10

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: microvillus; membrane; cytoplasmic microtubule; nucleus; brush border

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; L-xylulose reductase activity

Biological Process: glucose metabolic process; xylulose metabolic process; D-xylose metabolic process; NADP metabolic process; protein homotetramerization

Disease: Pentosuria