DCXR recombinant protein

DCXR Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121722

• Gene Names: DCXR; XR; DCR; HCR2; P34H; HCRII; KIDCR; PNTSU; SDR20C1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: DCXR; DCXR Protein; Human; Recombinant (His Tag); Human DCXR/HCR2 Protein (His Tag); DCR Protein; HCR2 Protein; HCRII Protein; KIDCR Protein; P34H Protein; PNTSU Protein; SDR20C1 Protein; XR Protein; dicarbonyl/L-xylulose reductase; DCXR recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 20% Glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys244

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human DCXR (Q7Z4W1)(Met1-Cys244) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for DCXR recombinant protein

Background: DCXR, also known as HCR2, belongs to the short-chain dehydrogenases/reductases (SDR) family. It is highly expressed in kidney, liver and epididymis. In the epididymis, DCXR is mainly expressed in the proximal and distal sections of the corpus region. HCR2 is weakly or not expressed in brain, lung, heart, spleen and testis. DCXR catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L-xylulose. DCXR participates in the uronate cycle of glucose metabolism. It may play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules.

References

Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40. Pierce SB, et al. (2011) Garrod's fourth inborn error of metabolism solved by the identification of mutations causing pentosuria. Proc Natl Acad Sci. 108(45):18313-7. Udeshi ND, et al. (2012) Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. Mol Cell Proteomics. 11(5):148-59.

NCBI and Uniprot Product Information

• NCBI GI #: 50400451
• NCBI GeneID: 51181
• UniProt Accession #: Q7Z4W1; Q9BTZ3; Q9UHY9
• Molecular Weight: 244
• NCBI Official Full Name: L-xylulose reductase
• NCBI Official Synonym Full Names: dicarbonyl/L-xylulose reductase
• NCBI Official Symbol: DCXR
• NCBI Official Synonym Symbols: XR; DCR; HCR2; P34H; HCRII; KIDCR; PNTSU; SDR20C1
• NCBI Protein Information: L-xylulose reductase; carbonyl reductase 2; carbonyl reductase II; sperm surface protein P34H; kidney dicarbonyl reductase; short chain dehydrogenase/reductase family 20C, member 1
• UniProt Protein Name: L-xylulose reductase
• UniProt Gene Name: DCXR
• UniProt Synonym Gene Names: XR; kiDCR
• UniProt Entry Name: DCXR_HUMAN

NCBI Description

The protein encoded by this gene acts as a homotetramer to catalyze diacetyl reductase and L-xylulose reductase reactions. The encoded protein may play a role in the uronate cycle of glucose metabolism and in the cellular osmoregulation in the proximal renal tubules. Defects in this gene are a cause of pentosuria. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2010]

Uniprot Description

DCXR: Catalyzes the NADPH-dependent reduction of several pentoses, tetroses, trioses, alpha-dicarbonyl compounds and L- xylulose. Participates in the uronate cycle of glucose metabolism. May play a role in the water absorption and cellular osmoregulation in the proximal renal tubules by producing xylitol, an osmolyte, thereby preventing osmolytic stress from occurring in the renal tubules. The enzyme defect in pentosuria has been shown to involve L-xylulose reductase. Essential pentosuria is an inborn error of metabolism characterized by the excessive urinary excretion of the pentose L-xylulose. Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Protein type: Oxidoreductase; Carbohydrate Metabolism - pentose and glucuronate interconversions; EC 1.1.1.10

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: microvillus; membrane; cytoplasmic microtubule; nucleus; brush border

Molecular Function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; L-xylulose reductase activity

Biological Process: glucose metabolic process; xylulose metabolic process; D-xylose metabolic process; NADP metabolic process; protein homotetramerization

Disease: Pentosuria