Western Blot (WB)
(Western blot analysis of DCTN4 using COLO205 whole cell lysates)
Rabbit DCTN4 Polyclonal Antibody | anti-DCTN4 antibody
DCTN4 Antibody
Gene Names
DCTN4; P62; DYN4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DCTN4; Polyclonal Antibody; DCTN4 Antibody; DCTN 4; Dctn4; DCTN4_HUMAN; Dynactin 4 (p62); Dynactin 4; Dynactin p62 subunit; Dynactin subunit 4; Dynactin subunit p62; Dynactin4; p62; anti-DCTN4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DCTN4 antibody detects endogenous levels of total DCTN4
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
467
Applicable Applications for anti-DCTN4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human DCTN4
Subcellular Location
Cytoplasm > Cytoskeleton. Cytoplasm > Cytoskeleton > Centrosome. Has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin. Overexpression does not disrupt microtubule organization or the integrity of the golgi but does cause both cytosolic and nuclear distribution, suggesting that this polypeptide may be targeted to the nucleus at very high expression levels.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of DCTN4 using COLO205 whole cell lysates)
Western Blot (WB)
(Western blot analysis of DCTN4 using COLO205 whole cell lysates)
Related Product Information for anti-DCTN4 antibody
Function: Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.
Subunit Structure: Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10 (By similarity). Binds directly to the ACTR1A subunit of dynactin. Interacts with ATP7B, but not ATP7A, in a copper-dependent manner. Interacts with ANK2; this interaction is required for localization at costameres (By similarity).
Similarity: Belongs to the dynactin subunit 4 family.
Subunit Structure: Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10 (By similarity). Binds directly to the ACTR1A subunit of dynactin. Interacts with ATP7B, but not ATP7A, in a copper-dependent manner. Interacts with ANK2; this interaction is required for localization at costameres (By similarity).
Similarity: Belongs to the dynactin subunit 4 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 52 kDa
Predicted: 53 kDa
Predicted: 53 kDa
NCBI Official Full Name
dynactin subunit 4 isoform a
NCBI Official Synonym Full Names
dynactin subunit 4
NCBI Official Symbol
DCTN4
NCBI Official Synonym Symbols
P62; DYN4
NCBI Protein Information
dynactin subunit 4
UniProt Protein Name
Dynactin subunit 4
Protein Family
UniProt Gene Name
DCTN4
UniProt Synonym Gene Names
Dyn4
Uniprot Description
Could have a dual role in dynein targeting and in ACTR1A/Arp1 subunit of dynactin pointed-end capping. Could be involved in ACTR1A pointed-end binding and in additional roles in linking dynein and dynactin to the cortical cytoskeleton.
Research Articles on DCTN4
Similar Products
Product Notes
The DCTN4 dctn4 (Catalog #AAA9609785) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DCTN4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DCTN4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the DCTN4 dctn4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCTN4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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