Rabbit anti-Human, Mouse TAF1 Polyclonal Antibody | anti-TAF1 antibody

Rabbit Polyclonal (IgG) to Human TAF1

Cat. Num. AAA247656

• Gene Names: TAF1; OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; N-TAF1; TAFII250; DYT3/TAF1; TAFII-250; TAF(II)250
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TAF1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TAF1; Anti-TAF1 Antibody (aa1131-1180) IHC-plus; BA2R; CCG1; CCGS; Cell cycle; G1 phase defect; CELL CYCLE GENE 1; Cell cycle gene 1 protein; Dystonia 3 (with Parkinsonism); DYT3; TAF(II)250; TBP Associated Factor 1; N-TAF1; NSCL2; p250; TAFII250; XDP; KAT4; TAF2A; TAFII-250; Human TAF1; anti-TAF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TAF1 Antibody detects endogenous levels of total TAF1 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1893

• Applicable Applications for anti-TAF1 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:10000), IHC-P (5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human TAF1 (aa1131-1180).
• Immunogen: TAF1 antibody was raised against synthetic peptide from human TAF1 (aa1131-1180).
• Antigen Modification: aa1131-1180
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-TAF1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Product Categories/Family for anti-TAF1 antibody

Family: Protein Kinase; Subfamily: TAF1

NCBI and Uniprot Product Information

• NCBI GI #: 20357585
• NCBI GeneID: 6872
• NCBI Accession #: NP_004597.2
• NCBI GenBank Nucleotide #: NM_004606.4
• UniProt Accession #: P21675; Q59FZ3; Q6IUZ1; Q70Q86; Q70Q87; Q70T00; Q70T01; A5CVC8; A5CVC9; A5CVD0; A5CVD1; B1Q2X3
• Molecular Weight: 214,913 Da
• NCBI Official Full Name: transcription initiation factor TFIID subunit 1 isoform 1
• NCBI Official Synonym Full Names: TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 250kDa
• NCBI Official Symbol: TAF1
• NCBI Official Synonym Symbols: OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; N-TAF1; TAFII250; DYT3/TAF1; TAFII-250; TAF(II)250
• NCBI Protein Information: transcription initiation factor TFIID subunit 1; cell cycle gene 1 protein; cell cycle, G1 phase defect; TBP-associated factor 250 kDa; transcription factor TFIID p250 polypeptide; complementation of cell cycle block, G1-to-S
• UniProt Protein Name: Transcription initiation factor TFIID subunit 1
• Protein Family: Taz1-interacting factor
• UniProt Gene Name: TAF1
• UniProt Synonym Gene Names: BA2R; CCG1; CCGS; TAF2A; p250; TAF(II)250; TAFII-250; TAFII250
• UniProt Entry Name: TAF1_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]

Uniprot Description

TAF1: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA- binding activity. Essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3); also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease. Belongs to the TAF1 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; EC 2.7.11.1; Protein kinase, atypical; EC 2.3.1.48; DNA-binding; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; TAF1 family

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function: protein serine/threonine kinase activity; protein binding; histone acetyltransferase activity; TATA-binding protein binding; sequence-specific DNA binding; p53 binding; transcription coactivator activity; transcription factor binding; ATP binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; protein amino acid autophosphorylation; transcription initiation; peptidyl-threonine phosphorylation; transcriptional preinitiation complex assembly; cell cycle; peptidyl-serine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; histone acetylation; response to DNA damage stimulus

Disease: Dystonia 3, Torsion, X-linked