Rabbit DcR3 Polyclonal Antibody

DcR3, NT (TR6, Decoy Receptor 3, TNFRSF6B, TR6, M68)

Cat. Num. AAA620825

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Highly Purified; Purified by ion exchange chromatography.
• Synonyms: DcR3; Polyclonal Antibody; NT (TR6; Decoy Receptor 3; TNFRSF6B; TR6; M68); Anti -DcR3; anti-DcR3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the N-terminus of human DcR3, otherwise known as Decoy Receptor 3, at ~33kD. Species crossreactivity: mouse and rat.
• Purity/Purification: Highly Purified; Purified by ion exchange chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-DcR3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-1ug/ml; Immunohistochemistry (Paraffin): 1ug/ml. Requires heat induced epitope retrieval. Sodium citrate buffer pH 6.0 is recommended for this purpose.
• Immunogen: Synthetic peptide corresponding to amino acids near the N-terminus of human DcR3 precursor.
• Positive Control: WB: Human heart lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemistry staining of human heart with MBS620825.)

Related Product Information for anti-DcR3 antibody

Decoy Receptor 3 is a 33kD member of the tumor necrosis factor receptor superfamily. DcR3 acts as a receptor for FasL and LIGHT, preventing them from binding to their cell death receptors and thereby inhibiting apoptosis. DcR3 is a soluble decoy receptor found in adult stomach, spinal cord, lymph node, trachea, spleen and lung. It may also be found in fetal lung, brain and liver. It is highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.

Product Categories/Family for anti-DcR3 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 37545624
• NCBI Official Full Name: decoy receptor 3 variant 2

NCBI Description

This gene belongs to the tumor necrosis factor receptor superfamily. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death. It acts as a decoy receptor that competes with death receptors for ligand binding. Over-expression of this gene has been noted in gastrointestinal tract tumors. Read-through transcription into this gene from the neighboring upstream gene, which encodes regulator of telomere elongation helicase 1 (RTEL1), generates a non-coding transcript. [provided by RefSeq, Feb 2011]

Uniprot Description

TNFRSF6B: Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis. Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: extracellular space

Molecular Function: protein binding; receptor activity

Biological Process: apoptosis; negative regulation of apoptosis