Goat DCN / Decorin Polyclonal Antibody | anti-DCN antibody

Goat Polyclonal to Human DCN / Decorin

Cat. Num. AAA244947

• Gene Names: DCN; CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human, Monkey; Predicted Reactivity: Pig (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: DCN / Decorin; Polyclonal Antibody; Goat Polyclonal to Human DCN / Decorin; Anti-DCN / Decorin Antibody (Internal) IHC-plus; DCN; CSCD; Decorin proteoglycan; PG-S2; PG40; Proteoglycan core protein; SLRR1B; Small leucine-rich protein 1B; PGII; PGS2; Bone proteoglycan II; Decorin; DSPG2; Human DCN; anti-DCN antibody

• Host: Goat
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human, Monkey; Predicted Reactivity: Pig (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human DCN / Decorin. This antibody is expected to recognize the three reported isoforms a, b and c (NP_001911.1; NP_598011.1; NP_598012.1 resp.). Reported variants NP_598010.1 and NP_001911.1 represent identical protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 359

• Applicable Applications for anti-DCN antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:8000), IHC-P (3.75 ug/ml), WB (0.5 - 2 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 38kDa band observed in Human Kidney lysates (calculated MW of 39.7kDa according to NP_001911.1). Recommended concentration: 0.5-2 ug/ml.
• Target Species: Human
• Immunogen Description: Synthetic peptide C-KISRVDAASLKGLNN from an internal region of human DCN / Decorin (NP_001911.1; NP_598011.1; NP_598012.1;).
• Immunogen Type: Synthetic peptide
• Immunogen: DCN / Decorin antibody was raised against synthetic peptide C-KISRVDAASLKGLNN from an internal region of human DCN / Decorin (NP_001911.1; NP_598011.1; NP_598012.1;). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%); Pig (93%); Marmoset, Sheep, Goat, Bat, Bovine, Guinea pig (87%); Elephant, Dog, Horse (80%).
• Antigen Modification: Internal
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-Decorin antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)

Western Blot (WB)

(DCN antibody (0.5 ug/ml) staining of Human Kidney lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4503271
• NCBI GeneID: 1634
• NCBI Accession #: NP_001911.1
• NCBI GenBank Nucleotide #: NM_001920.3
• UniProt Accession #: P07585; Q9P0Z0; Q9P0Z1; Q9Y5N8; Q9Y5N9
• Molecular Weight: 8,266 Da
• NCBI Official Full Name: decorin isoform a preproprotein
• NCBI Official Synonym Full Names: decorin
• NCBI Official Symbol: DCN
• NCBI Official Synonym Symbols: CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• NCBI Protein Information: decorin; PG-S2; bone proteoglycan II; decorin proteoglycan; proteoglycan core protein; small leucine-rich protein 1B; dermatan sulphate proteoglycans II
• UniProt Protein Name: Decorin
• Protein Family: Decorin
• UniProt Gene Name: DCN
• UniProt Synonym Gene Names: SLRR1B
• UniProt Entry Name: PGS2_HUMAN

NCBI Description

The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Extracellular matrix; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; lysosomal lumen; Golgi lumen; extracellular region; collagen type VI

Molecular Function: collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding

Biological Process: extracellular matrix organization and biogenesis; skeletal muscle development; chondroitin sulfate biosynthetic process; wound healing; glycosaminoglycan metabolic process; response to lipopolysaccharide; pathogenesis; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; extracellular matrix disassembly; organ morphogenesis; response to mechanical stimulus; carbohydrate metabolic process; chondroitin sulfate catabolic process; kidney development; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; placenta development; aging

Disease: Corneal Dystrophy, Congenital Stromal