Western Blot (WB)
(Western blot analysis of DCN antibody (Center) (MBS9209128) in mouse heart tissue lysates (35ug/lane). DCN (arrow) was detected using the purified Pab.)
Rabbit DCN Polyclonal Antibody | anti-DCN antibody
DCN Antibody (Center)
IHC-P~~1:10~50
FC~~1:10~50
Western Blot (WB)
(Western blot analysis of DCN antibody (Center) (MBS9209128) in mouse heart tissue lysates (35ug/lane). DCN (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DCN antibody (Center) (MBS9209128) in mouse heart tissue lysates (35ug/lane). DCN (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DCN (arrow) using rabbit polyclonal DCN Antibody (Center) (MBS9209128). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DCN gene.)
Western Blot (WB)
(Western blot analysis of DCN (arrow) using rabbit polyclonal DCN Antibody (Center) (MBS9209128). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DCN gene.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with DCN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma with DCN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC)
(DCN Antibody (Center) (MBS9209128) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC)
(DCN Antibody (Center) (MBS9209128) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a small cellular or pericellular matrix proteoglycan that is closely related in structure to biglycan protein. The encoded protein and biglycan are thought to be the result of a gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. It contains one attached glycosaminoglycan chain. This protein is capable of suppressing the growth of various tumor cell lines. There are multiple alternatively spliced transcript variants known for this gene. This gene is a candidate gene for Marfan syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis; Secreted
Chromosomal Location of Human Ortholog: 12q21.33
Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; proteinaceous extracellular matrix; Golgi lumen; extracellular region; collagen type VI
Molecular Function: collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding
Biological Process: extracellular matrix organization and biogenesis; skeletal muscle development; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; wound healing; response to lipopolysaccharide; pathogenesis; dermatan sulfate biosynthetic process; chondroitin sulfate metabolic process; extracellular matrix disassembly; organ morphogenesis; response to mechanical stimulus; carbohydrate metabolic process; chondroitin sulfate catabolic process; kidney development; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; aging; placenta development
Disease: Corneal Dystrophy, Congenital Stromal
Research Articles on DCN
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Product Notes
The DCN dcn (Catalog #AAA9209128) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 122-150. The DCN Antibody (Center) reacts with Human, Mouse (Predicted: Bovine, Pig, Sheep) and may cross-react with other species as described in the data sheet. AAA Biotech's DCN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS). WB~~1:1000 IHC-P~~1:10~50 FC~~1:10~50. Researchers should empirically determine the suitability of the DCN dcn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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