Rabbit anti-Human DCLK2 Polyclonal Antibody | anti-DCLK2 antibody

DCLK2, NT (DCLK2, DCAMKL2, DCDC3B, DCK2, Serine/threonine-protein kinase DCLK2, CaMK-like CREB regulatory kinase 2, Doublecortin domain-containing protein 3B, Doublecortin-like and CAM kinase-like 2, Doublecortin-like kinase 2) (FITC)

Cat. Num. AAA6291104

• Gene Names: DCLK2; CL2; DCK2; CLIK2; DCDC3; CLICK2; DCDC3B; DCAMKL2; CLICK-II
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DCLK2; Polyclonal Antibody; NT (DCLK2; DCAMKL2; DCDC3B; DCK2; Serine/threonine-protein kinase DCLK2; CaMK-like CREB regulatory kinase 2; Doublecortin domain-containing protein 3B; Doublecortin-like and CAM kinase-like 2; Doublecortin-like kinase 2) (FITC); Doublecortin-like kinase 2; anti-DCLK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DCLK2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DCLK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 167-196 amino acids from the N-terminal region of human DCLK2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DCLK2 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the DCLK2 antibody detected the DCLK2 protein (arrow).)

Related Product Information for anti-DCLK2 antibody

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified.

Product Categories/Family for anti-DCLK2 antibody

Antibodies; Protein Kinases

References

Dijkmans, T.F., et al. Cent Nerv Syst Agents Med Chem 10(1):32-46(2010). Kerjan, G., et al. Proc. Natl. Acad. Sci. U.S.A. 106(16):6766-6771(2009). Gorelik, A., et al. Cell. Mol. Biol. Lett. 13(4):614-620(2008). Tuy, F.P., et al. Dev. Neurosci. 30 (1-3), 171-186 (2008): Reiner, O., et al. BMC Genomics 7, 188 (2006).

NCBI and Uniprot Product Information

• NCBI GI #: 156713430
• NCBI GeneID: 166614
• NCBI Accession #: NP_001035351.3
• NCBI GenBank Nucleotide #: NM_001040261.3
• UniProt Accession #: Q8N568; Q59GC8; Q8N399; C9J5Q9
• Molecular Weight: 85,384 Da
• NCBI Official Full Name: serine/threonine-protein kinase DCLK2 isoform b
• NCBI Official Synonym Full Names: doublecortin like kinase 2
• NCBI Official Symbol: DCLK2
• NCBI Official Synonym Symbols: CL2; DCK2; CLIK2; DCDC3; CLICK2; DCDC3B; DCAMKL2; CLICK-II
• NCBI Protein Information: serine/threonine-protein kinase DCLK2
• UniProt Protein Name: Serine/threonine-protein kinase DCLK2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: DCLK2
• UniProt Synonym Gene Names: DCAMKL2; DCDC3B; DCK2; CL2; CLICK-II; CLICK2
• UniProt Entry Name: DCLK2_HUMAN

NCBI Description

This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010]

Uniprot Description

DCAMKL2: Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; DCAMKL family

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: cytoskeleton; cytoplasm

Molecular Function: protein serine/threonine kinase activity; ATP binding

Biological Process: protein amino acid phosphorylation