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Western Blot (WB) (Western Blot analysis of HepG2 cells using DCI Polyclonal Antibody)

Rabbit anti-Human DCI Polyclonal Antibody | anti-DCI antibody

DCI Polyclonal Antibody

Gene Names
ECI1; DCI
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
DCI; Polyclonal Antibody; DCI Polyclonal Antibody; ECI1; Enoyl-CoA delta isomerase 1; mitochondrial; 3; 2-trans-enoyl-CoA isomerase; Delta(3); Delta(2)-enoyl-CoA isomerase; D3; D2-enoyl-CoA isomerase; Dodecenoyl-CoA isomerase; anti-DCI antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
DCI Polyclonal Antibody detects endogenous levels of DCI protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
285
Applicable Applications for anti-DCI antibody
Western Blot (WB), ELISA (EIA)
Application Notes
Western Blot: 1:500 - 1:2000
ELISA: 1:20000
Not yet tested in other applications.
Immunogen Type
peptide
Immunogen Description
Synthesized peptide derived from the C-terminal region of human DCI.
Target Name
DCI
Preparation and Storage
Store at -20 degree C/1 year

Western Blot (WB)

(Western Blot analysis of HepG2 cells using DCI Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of HepG2 cells using DCI Polyclonal Antibody)
Product Categories/Family for anti-DCI antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,896 Da
NCBI Official Full Name
enoyl-CoA delta isomerase 1, mitochondrial isoform 2
NCBI Official Synonym Full Names
enoyl-CoA delta isomerase 1
NCBI Official Symbol
ECI1
NCBI Official Synonym Symbols
DCI
NCBI Protein Information
enoyl-CoA delta isomerase 1, mitochondrial
UniProt Protein Name
Enoyl-CoA delta isomerase 1, mitochondrial
UniProt Gene Name
ECI1
UniProt Synonym Gene Names
DCI; D3,D2-enoyl-CoA isomerase
UniProt Entry Name
ECI1_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]

Uniprot Description

DCI: Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Belongs to the enoyl-CoA hydratase/isomerase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Isomerase; Lipid Metabolism - fatty acid; EC 5.3.3.8; Mitochondrial

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane

Molecular Function: dodecenoyl-CoA delta-isomerase activity; intramolecular oxidoreductase activity

Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process

Research Articles on DCI

Similar Products

Product Notes

The DCI eci1 (Catalog #AAA9406398) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DCI Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DCI can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Western Blot: 1:500 - 1:2000 ELISA: 1:20000 Not yet tested in other applications. Researchers should empirically determine the suitability of the DCI eci1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DCI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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