Rabbit DCC Polyclonal Antibody | anti-DCC antibody

Anti-DCC antibody

Cat. Num. AAA176250

• Gene Names: DCC; CRC18; CRCR1; MRMV1; IGDCC1; NTN1R1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: DCC; Polyclonal Antibody; Anti-DCC antibody; Netrin receptor DCC; deleted in colorectal carcinoma; Colorectal cancer related chromosome sequence 18 antibody; Colorectal cancer suppressor antibody; CRC 18 antibody; CRC18 antibody; CRCR 1 antibody; CRCR1 antibody; DCC antibody; DCC_HUMAN antibody; Deleted in colorectal cancer protein antibody; Deleted in colorectal carcinoma antibody; Immunoglobulin superfamily DCC subclass member 1 antibody; Netrin receptor DCC antibody; Tumor suppressor protein DCC antibody; anti-DCC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Liquid; Contains 20mM PBS, 50% glycerol, 0.02% NaN3
• Concentration: 200ug/ml (varies by lot)
• Sequence Length: 1447

• Applicable Applications for anti-DCC antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot: ; Concentration: 0.1-0.5ug/ml; Tested Species: Hu, Ms, Rat; Predicted Species: -; Antigen Retrieval: -; ; Immunohistochemistry (Parafin-embedded Section):; Tested Species: 0.5-1ug/ml; Tested Species: Hu; Predicted Species: Ms, Rat; Antigen Retrieval: By Heat; ; Tested Species: In-house tested species with positive results. ; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins si required for the staining of formalin/paraffin sections.; ; Other applications have not been tested. Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DCC (162-178aa EVIGEPMPTIHWQKNQQ), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
• Preparation and Storage: At -20°C for 1 year, 4°C for one month. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-DCC antibody, MBS176250, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Mouse Brain Tissue LysateLane 3: U87 Cell LysateLane 4: SW620 Cell LysateLane 5: COLO320 Cell LysateLane 6: 293T Cell LysateLane 7: HELA Cell Lysate )

Immunohistochemistry (IHC)

(Anti-DCC antibody, MBS176250, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Related Product Information for anti-DCC antibody

DCC(Deleted IN Colorectal Carcinoma) also known as CRC18 or CRCR1. The DCC gene encodes a functional receptor for netrin and mediates axon outgrowth and the steering response. Heterozygous loss-of-function mutations in DCC can result in congenital mirror movements. Alterations in DCC occur frequently in colorectal cancer. Studying a YAC contig containing the entire DCC coding region, they showed that the DCC gene spans approximately 1.4 Mb. Vogelstein(1995) stated that the precise location of the DCC gene was though to be 18q21.3. DCC is a receptor or a component of a receptor that mediates the effects of netrin-1 on commissural axons, and they complement genetic evidence for interactions between DCC and netrin homologs in C. DCC protein could be detected in varying abundance in all specimens of normal colonic mucosa analyzed as well as in all specimens of adenomatous polyps, colorectal carcinoma and colorectal liver metastases. DCC may function as a tumor-suppressor protein by inducing apoptosis in settings in which ligand is unavailable(for example, during metastasis or tumor growth beyond local blood supply) through functional caspase cascades by a mechanism that requires cleavage of DCC at asp1290. Stein et al.(2001) concluded that DCC plays a central role in netrin signaling of axon growth and guidance independent of A2B receptor activation.

NCBI and Uniprot Product Information

• NCBI GI #: 296434474
• NCBI GeneID: 1630
• UniProt Accession #: P43146
• NCBI Official Full Name: Netrin receptor DCC
• NCBI Official Synonym Full Names: DCC netrin 1 receptor
• NCBI Official Symbol: DCC
• NCBI Official Synonym Symbols: CRC18; CRCR1; MRMV1; IGDCC1; NTN1R1
• NCBI Protein Information: netrin receptor DCC; colorectal tumor suppressor; colorectal cancer suppressor; tumor suppressor protein DCC; deleted in colorectal carcinoma; deleted in colorectal cancer protein; immunoglobulin superfamily DCC subclass member 1; immunoglobulin superfamily, DCC subclass, member 1
• UniProt Protein Name: Netrin receptor DCC
• Protein Family: DCC family protein
• UniProt Gene Name: DCC
• UniProt Synonym Gene Names: IGDCC1
• UniProt Entry Name: DCC_HUMAN

NCBI Description

This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma. [provided by RefSeq, Oct 2009]

Uniprot Description

DCC: Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene. Defects in DCC are the cause of mirror movements type 1 (MRMV1). A disorder characterized by contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities. Belongs to the immunoglobulin superfamily. DCC family.

Protein type: Immunoglobulin superfamily; Tumor suppressor; Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q21.3

Cellular Component: axon; integral to membrane; plasma membrane; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; netrin receptor activity; transmembrane receptor activity; transcription coactivator activity

Biological Process: axon guidance; axonogenesis; spinal cord ventral commissure morphogenesis; positive regulation of apoptosis; apoptosis; response to amphetamine; neuron migration; negative regulation of collateral sprouting; dorsal/ventral axon guidance; anterior/posterior axon guidance

Disease: Mirror Movements 1; Esophageal Cancer; Colorectal Cancer