Rabbit DCC Polyclonal Antibody | anti-DCC antibody

DCC (F465) Polyclonal Antibody

Cat. Num. AAA3003535

• Gene Names: DCC; CRC18; CRCR1; MRMV1; HGPPS2; IGDCC1; NTN1R1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DCC; Polyclonal Antibody; DCC (F465) Polyclonal Antibody; Netrin receptor DCC; Colorectal cancer suppressor; Immunoglobulin superfamily DCC subclass member 1; Tumor suppressor protein DCC; IGDCC1; anti-DCC antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DCC (F465) polyclonal antibody detects endogenous levels of DCC protein
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1447

• Applicable Applications for anti-DCC antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 450-500 of Human DCC.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Related Product Information for anti-DCC antibody

DCC (deleted in colorectal cancer) was first identified as a candidate tumor suppressor gene based on its absence or reduced expression in the majority of colorectal cancers. Loss of DCC expression was subsequently observed in cancers of the breast, endometrium, brain, pancreas and prostate, as well as in leukemias, neuroblastomas and male germ cell cancers. DCC is a 1447 amino acid transmembrane protein with highest expression in developing brain and neural tube and is suspected to play a role in mediating directional migration in the developing nervous system. Netrin-1, a chemoattractant for commissural axons in the spinal cord, has been identified as a ligand for DCC.

NCBI and Uniprot Product Information

• NCBI GI #: 110431348
• NCBI GeneID: 1630
• NCBI Accession #: NP_005206.2
• NCBI GenBank Nucleotide #: NP_005206.2
• UniProt Accession #: P43146
• Molecular Weight: ~ 158kDa
• NCBI Official Full Name: netrin receptor DCC
• NCBI Official Synonym Full Names: DCC netrin 1 receptor
• NCBI Official Symbol: DCC
• NCBI Official Synonym Symbols: CRC18; CRCR1; MRMV1; HGPPS2; IGDCC1; NTN1R1
• NCBI Protein Information: netrin receptor DCC
• UniProt Protein Name: Netrin receptor DCC
• Protein Family: DCC family protein
• UniProt Gene Name: DCC
• UniProt Synonym Gene Names: IGDCC1
• UniProt Entry Name: DCC_HUMAN

NCBI Description

This gene encodes a netrin 1 receptor. The transmembrane protein is a member of the immunoglobulin superfamily of cell adhesion molecules, and mediates axon guidance of neuronal growth cones towards sources of netrin 1 ligand. The cytoplasmic tail interacts with the tyrosine kinases Src and focal adhesion kinase (FAK, also known as PTK2) to mediate axon attraction. The protein partially localizes to lipid rafts, and induces apoptosis in the absence of ligand. The protein functions as a tumor suppressor, and is frequently mutated or downregulated in colorectal cancer and esophageal carcinoma. [provided by RefSeq, Oct 2009]

Uniprot Description

DCC: Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene. Defects in DCC are the cause of mirror movements type 1 (MRMV1). A disorder characterized by contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities. Belongs to the immunoglobulin superfamily. DCC family.

Protein type: Membrane protein, integral; Immunoglobulin superfamily; Tumor suppressor; Receptor, misc.

Chromosomal Location of Human Ortholog: 18q21.3

Cellular Component: axon; integral to membrane; plasma membrane; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; transmembrane receptor activity; netrin receptor activity; transcription coactivator activity

Biological Process: axon guidance; axonogenesis; spinal cord ventral commissure morphogenesis; positive regulation of apoptosis; apoptosis; response to amphetamine; neuron migration; negative regulation of collateral sprouting; dorsal/ventral axon guidance; anterior/posterior axon guidance

Disease: Mirror Movements 1; Esophageal Cancer; Colorectal Cancer