Rabbit anti-Human, mouse DCBLD2 Polyclonal Antibody | anti-DCBLD2 antibody

DCBLD2 Antibody (C-term)

Cat. Num. AAA9205420

• Gene Names: DCBLD2; ESDN; CLCP1
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DCBLD2; Polyclonal Antibody; DCBLD2 Antibody (C-term); Discoidin; CUB and LCCL domain-containing protein 2; CUB; LCCL and coagulation factor V/VIII-homology domains protein 1; Endothelial and smooth muscle cell-derived neuropilin-like protein; CLCP1; ESDN; anti-DCBLD2 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DCBLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 538-567 amino acids from the C-terminal region of human DCBLD2.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 538-567
• Sequence Length: 775

• Applicable Applications for anti-DCBLD2 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(DCBLD2 Antibody (C-term) western blot analysis in mouse NIH-3T3,Neuro-2a cell line and mouse stomach tissue and U251,A2058,A375,Sk-Br-3,T47D cell line lysates (35ug/lane).This demonstrates the DCBLD2 antibody detected the DCBLD2 protein (arrow).)

Flow Cytometry (FC/FACS)

(DCBLD2 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DCBLD2 antibody

DCBLD2, otherwise known as ESDN (endothelial and smooth muscle cell-derived neuropilin-like molecule) is a novel type-I transmembrane protein with the longest cleavable secretory signal sequence among eukaryotes. It is expressed in various tissues; particularly highly expressed in cultured vascular smooth muscle cells. DCBLD2 is considered to play a role in regulation of vascular cell growth and may have a wide variety of functions in other tissues including the nervous system, like neuropilins.

Product Categories/Family for anti-DCBLD2 antibody

Cancer

References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Guo, X., et al. J. Biol. Chem. 284(43):29376-29382(2009)
Marroni, F., et al. Circ Cardiovasc Genet 2(4):322-328(2009)
Kim, M., et al. Mol. Cancer Res. 6(2):222-230(2008)
Sadeghi, M.M., et al. Am. J. Transplant. 7(9):2098-2105(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 54792129
• NCBI GeneID: 131566
• NCBI Accession #: NP_563615.3
• NCBI GenBank Nucleotide #: NM_080927.3
• UniProt Accession #: Q96PD2; Q8N6M4; Q8TDX2; B7WNL1; D3DN41
• Molecular Weight: 85035
• NCBI Official Full Name: discoidin, CUB and LCCL domain-containing protein 2
• NCBI Official Synonym Full Names: discoidin, CUB and LCCL domain containing 2
• NCBI Official Symbol: DCBLD2
• NCBI Official Synonym Symbols: ESDN; CLCP1
• NCBI Protein Information: discoidin, CUB and LCCL domain-containing protein 2
• UniProt Protein Name: Discoidin, CUB and LCCL domain-containing protein 2
• Protein Family: Discoidin, CUB and LCCL domain-containing protein
• UniProt Gene Name: DCBLD2
• UniProt Synonym Gene Names: CLCP1; ESDN
• UniProt Entry Name: DCBD2_HUMAN

Uniprot Description

DCBLD2: a type-I transmembrane protein. May be involved in vascular remodeling and influence vascular smooth muscle cell proliferation. Contains a CUB domain and a coagulation factor V/VIII homology domain, similar to the structure of the neuropilins. Plays a role in cell motility. SEMA4B appears to be one of its ligands. Strongly expressed in nerve bundles. Highly expressed in testis, heart, skeletal muscle and also in cultured vascular smooth muscle cells. Increased in lung cancers during the process of tumor progression. A target of EGF signaling in the A431 cervical cancer cell-line. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q12.1|3

Cellular Component: cell surface; integral to plasma membrane

Molecular Function: protein binding

Biological Process: intracellular receptor-mediated signaling pathway; wound healing; negative regulation of cell growth