Rabbit anti-Human, Mouse DCBLD2 Polyclonal Antibody | anti-DCBLD2 antibody

DCBLD2, CT (DCBLD2, CLCP1, ESDN, Discoidin, CUB and LCCL domain-containing protein 2, CUB, LCCL and coagulation factor V/VIII-homology domains protein 1, Endothelial and smooth muscle cell-derived neuropilin-like protein)

Cat. Num. AAA6012724

• Gene Names: DCBLD2; ESDN; CLCP1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DCBLD2; Polyclonal Antibody; CT (DCBLD2; CLCP1; ESDN; Discoidin; CUB and LCCL domain-containing protein 2; CUB; LCCL and coagulation factor V/VIII-homology domains protein 1; Endothelial and smooth muscle cell-derived neuropilin-like protein); Anti -DCBLD2; anti-DCBLD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-DCBLD2 antibody: ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50
• Immunogen: DCBLD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-567 amino acids from the C-terminal region of human DCBLD2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DCBLD2 Antibody (C-term) western blot analysis in mouse NIH-3T3,Neuro-2a cell line and mouse stomach tissue and U251,A2058,A375,Sk-Br-3,T47D cell line lysates (35ug/lane).This demonstrates the DCBLD2 antibody detected the DCBLD2 protein (arrow).)

Flow Cytometry (FC/FACS)

(DCBLD2 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-DCBLD2 antibody

DCBLD2, otherwise known as ESDN (endothelial and smooth muscle cell-derived neuropilin-like molecule) is a novel type-I transmembrane protein with the longest cleavable secretory signal sequence among eukaryotes. It is expressed in various tissues; particularly highly expressed in cultured vascular smooth muscle cells. DCBLD2 is considered to play a role in regulation of vascular cell growth and may have a wide variety of functions in other tissues including the nervous system, like neuropilins.

Product Categories/Family for anti-DCBLD2 antibody

Antibodies; Abs to Coagulation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 54792129
• NCBI GeneID: 131566
• NCBI Accession #: NP_563615.3
• NCBI GenBank Nucleotide #: NM_080927.3
• UniProt Accession #: Q96PD2; Q8N6M4; Q8TDX2; B7WNL1; D3DN41
• Molecular Weight: 85,035 Da
• NCBI Official Full Name: discoidin, CUB and LCCL domain-containing protein 2
• NCBI Official Synonym Full Names: discoidin, CUB and LCCL domain containing 2
• NCBI Official Symbol: DCBLD2
• NCBI Official Synonym Symbols: ESDN; CLCP1
• NCBI Protein Information: discoidin, CUB and LCCL domain-containing protein 2; 1700055P21Rik; coagulation factor V/VIII-homology domains protein 1; CUB, LCCL and coagulation factor V/VIII-homology domains protein 1; endothelial and smooth muscle cell-derived neuropilin-like protein
• UniProt Protein Name: Discoidin, CUB and LCCL domain-containing protein 2
• Protein Family: Discoidin, CUB and LCCL domain-containing protein
• UniProt Gene Name: DCBLD2
• UniProt Synonym Gene Names: CLCP1; ESDN
• UniProt Entry Name: DCBD2_HUMAN

Uniprot Description

DCBLD2: a type-I transmembrane protein. May be involved in vascular remodeling and influence vascular smooth muscle cell proliferation. Contains a CUB domain and a coagulation factor V/VIII homology domain, similar to the structure of the neuropilins. Plays a role in cell motility. SEMA4B appears to be one of its ligands. Strongly expressed in nerve bundles. Highly expressed in testis, heart, skeletal muscle and also in cultured vascular smooth muscle cells. Increased in lung cancers during the process of tumor progression. A target of EGF signaling in the A431 cervical cancer cell-line. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q12.1|3

Cellular Component: cell surface; integral to plasma membrane

Molecular Function: protein binding

Biological Process: intracellular receptor-mediated signaling pathway; wound healing; negative regulation of cell growth