Rabbit anti-Human DC-SIGN Polyclonal Antibody | anti-CD209 antibody

DC-SIGN, CT (Dendritic Cells, CD209, Dendritic Cell-Specific ICAM-3, SIGNR1, SIGN-R1)

Cat. Num. AAA6002714

• Gene Names: CD209; CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: DC-SIGN; Polyclonal Antibody; CT (Dendritic Cells; CD209; Dendritic Cell-Specific ICAM-3; SIGNR1; SIGN-R1); Anti -DC-SIGN; anti-CD209 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DC-SIGN at ~ 44kD.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-CD209 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western blot: 1-2ug/ml detects GST-DC-SIGN fusion protein expressed in E. coli in the absence and presence of inducing reagent IPTG with anti-DC-SIGN at 0.5ug/ml.; Immunohistochemistry: 10ug/ml
• Immunogen: Synthetic peptide (CSRDEEQFLSPAPATPN PPPA) corresponding to aa384 to 404 of human DC-SIGN, C-terminal (1).
• Positive Control: Human placenta lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot detection of GST-DC-SIGN fusion protein expressed in E.Coli in the absence (lane 1) and presence (lane 2) of inducing reagent IPTG with MBS6002714 at 0.5ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of MBS6002714.)

Related Product Information for anti-CD209 antibody

Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes (1), where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently (4-8). DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR (8). The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.

Product Categories/Family for anti-CD209 antibody

Antibodies; Abs to CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 13383468
• NCBI GeneID: 30835
• UniProt Accession #: Q9NNX6; Q2TB19; Q96QP7; Q96QP8; Q96QP9; Q96QQ0; Q96QQ1; Q96QQ2; Q96QQ3; A8KAM4; A8MVQ9; G5E9C4
• Molecular Weight: 45,775 Da
• NCBI Official Full Name: DC-SIGN
• NCBI Official Synonym Full Names: CD209 molecule
• NCBI Official Symbol: CD209
• NCBI Official Synonym Symbols: CDSIGN; CLEC4L; DC-SIGN; DC-SIGN1
• NCBI Protein Information: CD209 antigen; HIV gpl20-binding protein; C-type lectin domain family 4 member L; C-type lectin domain family 4, member L; dendritic cell-specific ICAM-3-grabbing non-integrin 1; dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin
• UniProt Protein Name: CD209 antigen
• Protein Family: CD209 antigen
• UniProt Gene Name: CD209
• UniProt Synonym Gene Names: CLEC4L; DC-SIGN; DC-SIGN1
• UniProt Entry Name: CD209_HUMAN

NCBI Description

This gene encodes a transmembrane receptor and is often referred to as DC-SIGN because of its expression on the surface of dendritic cells and macrophages. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are rare but have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 10332; often referred to as L-SIGN). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants.[provided by RefSeq, Feb 2009]

Uniprot Description

CD209: Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri- mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA. Homotetramer. Binds to many viral surface glycoproteins such as HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus envelope glycoproteins, cytomegalovirus gB, HCV E2 and dengue virus major envelope protein E. Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. 13 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: cell surface; membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: mannose binding; protein binding; peptide antigen binding; metal ion binding; virion binding; carbohydrate binding

Biological Process: heterophilic cell adhesion; cell-cell recognition; antigen processing and presentation; leukocyte adhesion; virus-host interaction; regulation of T cell proliferation; innate immune response; peptide antigen transport; endocytosis; viral genome replication; virion attachment to host cell surface receptor

Disease: Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To