Western Blot (WB)
(Western blot analysis of DARPP32 (pT75) expression in MDA-MB-361 (A), SHSY5Y (B) whole cell lysates.)
Rabbit DARPP32 Polyclonal Antibody | anti-PPP1R1B antibody
Anti-DARPP32 (pT75) Phospho Antibody
Western Blot (WB)
(Western blot analysis of DARPP32 (pT75) expression in MDA-MB-361 (A), SHSY5Y (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of DARPP32 (pT75) expression in MDA-MB-361 (A), SHSY5Y (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of DARPP32 (pT75) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of DARPP32 (pT75) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
DARPP-32: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.
Protein type: Protein phosphatase, regulatory subunit; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: cell soma; cytoplasm; nucleus; cytosol
Molecular Function: protein kinase inhibitor activity; protein phosphatase type 1 regulator activity; D4 dopamine receptor binding; protein phosphatase inhibitor activity; type 1 serine/threonine specific protein phosphatase inhibitor activity; D3 dopamine receptor binding; D1 dopamine receptor binding; D5 dopamine receptor binding; D2 dopamine receptor binding
Biological Process: negative regulation of female receptivity; transcription, DNA-dependent; regulation of catalytic activity; negative regulation of catalytic activity; response to amphetamine; visual learning; negative regulation of protein kinase activity; signal transduction
Research Articles on PPP1R1B
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Product Notes
The PPP1R1B ppp1r1b (Catalog #AAA8200609) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DARPP32 (pT75) Phospho Antibody reacts with Human, Mouse, Rat, Porcine and may cross-react with other species as described in the data sheet. AAA Biotech's DARPP32 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB (1/500 - 1/1000), IHC (1/100 - 1/200). Researchers should empirically determine the suitability of the PPP1R1B ppp1r1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DARPP32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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